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JoVE Journal Neuroscience
Ontleedende cel-autonome functie van fragiele X mentale retardatie-eiwit in een auditief circuit door In Ovo-elektroporatie
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Ontleedende cel-autonome functie van fragiele X mentale retardatie-eiwit in een auditief circuit door In Ovo-elektroporatie

Article DOI: 10.3791/64187-v • 11:10 min • July 6th, 2022
July 6th, 2022 •
*1, *2, 3, 1
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine, Jinan University, 2Department of Clinical Pathology, First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine, Florida State University
* These authors contributed equally

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Met behulp van ovo-elektroporatie hebben we een methode ontwikkeld om selectief het auditieve binnenoor en de cochleaire kern in kippenembryo's te transfecteren om een celgroepspecifieke knockdown van fragiele X mentale retardatie-eiwit te bereiken tijdens discrete perioden van circuitassemblage.

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Neurowetenschappen fragiele X-syndroom sensorisch circuit binnenoor cochleaire kern synapsvorming hersenontwikkeling endbulb van Held kippenembryo
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