Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

Jamie Snider; Saranya Kittanakom; Jasna Curak; Igor Stagljar

doi:10.3791/1698

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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

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生物学

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JoVE Journal 生物学

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

30,816 Views

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14:04 min

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February 01, 2010

DOI:

10.3791/1698-v

DOI:

10.3791/1698-v

•

14:04 min

February 01, 2010

•

7 Views

Jamie Snider^1,2,3, Saranya Kittanakom^1,2,3, Jasna Curak^1,2,3, Igor Stagljar^1,2,3

¹Department of Biochemistry,University of Toronto, ²Department of Molecular Genetics,University of Toronto, ³Terrence Donnelly Centre for Cellular and Biomolecular Research (CCBR),University of Toronto

概要

MYTH allows the sensitive detection of transient and stable interactions between proteins that are expressed in the model organism Saccharomyces cerevisiae. It has been successfully applied to study exogenous and yeast integral membrane proteins in order to identify their interacting partners in a high throughput manner.

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Split-ubiquitin Membrane Yeast Two-hybrid Protein-protein Interactions MYTH System Full-length Transmembrane Proteins Saccharomyces Cerevisiae Bait Protein Prey Proteins Ubiquitin Moieties Artificial Transcription Factor Pseudo-ubiquitin Molecule Deubiquitinating Enzymes Reporter Gene Expression

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Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions

概要

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