JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

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Improved UPLC-UV Method for the Quantification of Vitamin C in Lettuce Varieties (Lactuca sativa L.) and Crop Wild Relatives (Lactuca spp.)

Inés Medina-Lozano*^1,4, Juan Ramón Bertolín*^2,4, Raquel Zufiaurre³, Aurora Díaz^1,4

¹Unidad de Hortofruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ²Unidad de Producción y Sanidad Animal, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITA), ³Dpto Química Analítica, Escuela Politécnica Superior (Universidad de Zaragoza), ⁴Instituto Agroalimentario de Aragón - IA2 (CITA-Universidad de Zaragoza)

JoVE 61440

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC

Philipp Gaugler¹, Verena Gaugler¹, Marília Kamleitner¹, Gabriel Schaaf¹

¹Department of Plant Nutrition, Institute of Crop Science and Resource Conservation, University of Bonn

JoVE 61495

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis

Chuan-Chih Hsu^1,3, Chia-Feng Tsai², W. Andy Tao^3,4, Pengcheng Wang⁵

¹Department of Plant Biology, Carnegie Institute for Science, ²Graduate School of Pharmaceutical Sciences, Kyoto University, ³Department of Biochemistry, Purdue University, ⁴Department of Chemistry, Purdue University, ⁵Shanghai Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences

JoVE 61489

Non-Radioactive In Vitro Cardiac Myosin Light Chain Kinase Assays

Kenta Kamikubo*¹, Osamu Tsukamoto*¹, Yuki Uyama-Saito*¹, Ryohei Oya¹, Tomoya Tsubota¹, Noboru Fujino³, Yoshihiro Asano², Hisakazu Kato¹, Ken Matsuoka¹, Seiji Takashima¹

¹Department of Medical Biochemistry, Osaka University Graduate School of Medicine/Frontier Biosciences, ²Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine/Frontier Biosciences, ³Department of Cardiovascular and internal medicine, Kanazawa University Graduate School of Medicine

JoVE 61168

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Hyeoncheol Kim¹, Xiang Xue¹

¹Department of Biochemistry and Molecular Biology, University of New Mexico

JoVE 60682

Assessment of Cellular Oxidation using a Subcellular Compartment-Specific Redox-Sensitive Green Fluorescent Protein

Alev Tascioglu Aliyev^1,2, Francesca LoBianco¹, Kimberly J. Krager¹, Nukhet Aykin-Burns¹

¹Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, ²Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

JoVE 61229

Ubiquitin Chain Analysis by Parallel Reaction Monitoring

Joseph Longworth¹, Marta L. Mendes¹, Gunnar Dittmar¹

¹Quantitative Biology Unit, Luxembourg Institute of Health

JoVE 60702

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Silveli Suzuki-Hatano¹, Ang-Chen Tsai¹, Audrey Daugherty¹, Christina A. Pacak¹

¹Department of Pediatrics, University of Florida

JoVE 60970

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking

Santosh Adhikari¹, Chiranjib Banerjee*¹, Joe Moscatelli*^1,2, Elias M. Puchner¹

¹School of Physics and Astronomy, University of Minnesota, Twin Cities, Physics and Nanotechnology (PAN), ²Middlebury College

JoVE 60950

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay

Emily M. Chester¹, Emily Fender¹, Michael D. Wasserman¹

¹Department of Anthropology, Indiana University

JoVE 61005

A Planarian Motility Assay to Gauge the Biomodulating Properties of Natural Products

Evelyn B. Voura¹, Carlos H. Pulquerio¹, Robyn A. M. V. Fong¹, Zaina Imani¹, Pablo J. Rojas¹, Angela M. Pratt¹, Nicole M. Shantel¹, Elisa J. Livengood¹

¹State University of New York (SUNY) at Morrisville

JoVE 61070

Methods Collection

An Electrochemiluminescence-Based Assay for MeCP2 Protein Variants

Hannes Steinkellner¹, Alexander V. Beribisky¹, Philip Mausberg¹, John Christodoulou², Barbara Scheiber-Mojdehkar³, Anna Huber¹, Victoria Sarne¹, Franco Laccone¹

¹Institute of Medical Genetics, Center for Pathobiochemistry and Genetics, Medical University of Vienna (MUV), ²Murdoch Children's Research Institute and Department of Paediatrics, University of Melbourne, Discipline of Child & Adolescent Health, Sydney Medical School, ³Department of Medical Chemistry and Pathobiochemistry, Medical University of Vienna

JoVE 61054

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Vito M. Butardo Jr.¹, Christiane Seiler², Nese Sreenivasulu³, Markus Kuhlmann²

¹Department of Chemistry and Biotechnology, Swinburne University of Technology, ²Department of Molecular Genetics, Heterosis Research Group, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), ³Grain Quality and Nutrition Center, Applied Functional Genomics Cluster, International Rice Research Institute

JoVE 60602

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Mahlet Z. Tamirat¹, Kari J. Kurppa², Klaus Elenius^2,3,4, Mark S. Johnson¹

¹Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, ²Medicity Research Laboratories and Institute of Biomedicine, University of Turku, ³Turku Bioscience Centre, University of Turku and Åbo Akademi University, ⁴Department of Oncology and Radiotherapy, University of Turku and Turku University Hospital

JoVE 61125

X-Ray Crystallography to Study the Oligomeric State Transition of the Thermotoga maritima M42 Aminopeptidase TmPep1050

Raphael Dutoit^1,2, Nathalie Brandt², Dany Van Elder¹, Louis Droogmans¹

¹Laboratory of Microbiology, Department of Molecular Biology, Université Libre de Bruxelles, ²Labiris Institut de Recherche

JoVE 61288

Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening

Karine Nozeret¹, Aurélia Pernin¹, Nienke Buddelmeijer¹

¹Institut Pasteur, Unité Biologie et génétique de la paroi bactérienne, Paris, France ; CNRS, UMR 2001 « Microbiologie intégrative et Moléculaire », Paris, France ; INSERM, Équipe Avenir, Paris, France

JoVE 61146

Monitoring Protein-RNA Interaction Dynamics in vivo at High Temporal Resolution using χCRAC

Stuart W. McKellar¹, Ivayla Ivanova¹, Robert W. van Nues², Ross A. Cordiner³, Will Worboys⁴, Andrew Langford⁴, Torben Heick Jensen³, Sander Granneman¹

¹Centre for Synthetic and Systems Biology, University of Edinburgh, ²Institute of Cell Biology, University of Edinburgh, ³Department of Molecular Biology and Genetics, Aarhus University, ⁴UVO3 Ltd.