JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

1 2 3 4 5 6 7 8 9…12 › 272 VIDEO ARTICLES

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Stephanie Hutin¹, Bart Van Laer¹, Christoph Mueller-Dieckmann¹, Gordon Leonard¹, Didier Nurizzo¹, Matthew W. Bowler²

¹Structural Biology Group, European Synchrotron Radiation Facility, ²Grenoble Outstation, European Molecular Biology Laboratory

JoVE 59032

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Stephanie Hutin*¹, Gianluca Santoni*¹, Ulrich Zander², Nicolas Foos¹, Sylvain Aumonier¹, Guillaume Gotthard¹, Antoine Royant^1,3, Christoph Mueller-Dieckmann¹, Gordon Leonard¹

¹European Synchrotron Radiation Facility, Structural Biology Group, ²European Molecular Biology Laboratory, ³Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)

JoVE 58594

Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale

Melaku Garsamo^1,3, Yun Zhou^2,3, Xing Liu^1,3

¹Department of Biochemistry, Purdue University, ²Department of Botany and Plant Pathology, Purdue University, ³Center for Plant Biology, Purdue University

JoVE 59038

Expression, Solubilization, and Purification of Eukaryotic Borate Transporters

Sebastian Flores¹, Andrew P. Feld¹, Bryan H. Thurtle-Schmidt¹

¹Department of Biology, Davidson College

JoVE 59166

Quantification of Hypopigmentation Activity In Vitro

Yeon-Ji Kim¹, Min-Jung Kim¹, Dong-Keon Kweon², Seung-Taik Lim¹, Sung-Joon Lee¹

¹Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology, Korea University, ²JinWoo Bio Co. Ltd

JoVE 58185

Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle

Sujin Kim¹, Minjeong Kang¹, Yoosik Kim¹

¹Department of Chemical and Biomolecular Engineering and KI for Health Science and Technology (KIHST), Korea Advanced Institute of Science and Technology (KAIST)

JoVE 59215

Force Spectroscopy of Single Protein Molecules Using an Atomic Force Microscope

Zackary N. Scholl¹, Qing Li¹, Eric Josephs¹, Dimitra Apostolidou¹, Piotr E. Marszalek¹

¹Mechanical Engineering and Materials Science, Duke University

JoVE 55989

Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

Dominik P. Buser¹, Martin Spiess¹

¹Biozentrum, University of Basel

JoVE 59111

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

John E. Chojnowski¹, Emily A. McMillan¹, Todd I. Strochlic¹

¹Department of Biochemistry and Molecular Biology, Drexel University College of Medicine

JoVE 59037

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Ievgen Mazurenko¹, Nikos S. Hatzakis², Lars J.C. Jeuken¹

¹School of Biomedical Sciences & the Astbury Centre for Structural Molecular Biology, University of Leeds, ²Department of Chemistry and Nano-Science Center, University of Copenhagen

JoVE 58896

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Kentaro Ito¹, Bilge Argunhan¹, Hideo Tsubouchi¹, Hiroshi Iwasaki¹

¹Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology

JoVE 59073

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

Alex Kirui*¹, Malitha C. Dickwella Widanage*¹, Frederic Mentink-Vigier², Ping Wang³, Xue Kang¹, Tuo Wang¹

¹Department of Chemistry, Louisiana State University, ²National High Magnetic Field Laboratory, ³Departments of Pediatrics, and Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center

JoVE 59152

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Nicholas McCaul^1,2, Hui Ying Yeoh¹, Guus van Zadelhoff¹, Naomi Lodder¹, Bertrand Kleizen¹, Ineke Braakman¹

¹Cellular Protein Chemistry, Utrecht University, ²Program in Cellular and Molecular Medicine, Boston Children's Hospital

JoVE 58952

How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project

Daniel Bruce¹, Emily Cardew¹, Stefanie Freitag-Pohl², Ehmke Pohl^1,2

¹Department of Biosciences, Durham University, ²Department of Chemistry, Durham University

JoVE 58666

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Christophe Jung¹, Max Schnepf¹, Peter Bandilla¹, Ulrich Unnerstall¹, Ulrike Gaul¹

¹Gene Center and Department of Biochemistry, Center for Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität München

JoVE 58763

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli

Patrick Rosendahl Andreassen¹, Jens Sivkær Pettersen¹, Mikkel Jørgensen¹

¹Department of Biochemistry and Molecular Biology, University of Southern Denmark

JoVE 58996

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Micah P. Stumme-Diers¹, Tommy Stormberg¹, Zhiqiang Sun¹, Yuri L. Lyubchenko¹

¹Department of Pharmaceutical Sciences, University of Nebraska Medical Center

JoVE 58820

Immunization of Alpacas (Lama pacos) with Protein Antigens and Production of Antigen-specific Single Domain Antibodies

K. Martin Chow¹, Sidney W. Whiteheart¹, Jeffrey R. Smiley², Savita Sharma¹, Kathy Boaz², Meggie J. Coleman², Alvina Maynard³, Louis B. Hersh¹, Craig W. Vander Kooi¹

¹Department of Molecular and Cellular Biochemistry, University of Kentucky, ²Division of Laboratory Animal Resources, University of Kentucky, ³River Hill Ranch

JoVE 58471

Isolation of F₁-ATPase from the Parasitic Protist Trypanosoma brucei

Ondřej Gahura¹, Alena Zíková^1,2

¹Biology Centre, Czech Academy of Science, Institute of Parasitology, ²Faculty of Science, University of South Bohemia

JoVE 58334

Cell Fractionation of U937 Cells in the Absence of High-speed Centrifugation

William D. McCaig¹, Matthew A. Deragon¹, Robert J. Haluska Jr,¹, Alexa L. Hodges¹, Payal S. Patel¹, Timothy J. LaRocca¹

¹Department of Basic and Clinical Sciences, Albany College of Pharmacy and Health Sciences

JoVE 59022

Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

Eva Königshausen¹, Sebastian A. Potthoff¹, Raphael Haase¹, Catherine Meyer-Schwesinger², Ernest Kaufmann¹, L. Christian Rump¹, Johannes Stegbauer¹, Lorenz Sellin¹, Ivo Quack¹, Magdalena Woznowski¹

¹Department of Nephrology, Medical Faculty, Heinrich-Heine-University, ²Institute of Cellular and Integrative Physiology, University Medical Center Hamburg-Eppendorf

JoVE 58542

Use of Recombinant Fusion Proteins in a Fluorescent Protease Assay Platform and Their In-gel Renaturation

Beáta Bozóki*^1,2, János András Mótyán*¹, Márió Miczi¹, Lívia Diána Gazda¹, József Tőzsér¹

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, ²Biotechnology Research Department, Gedeon Richter Plc

JoVE 58824

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Hanan B. Elajaili¹, Laura Hernandez-Lagunas¹, Kalina Ranguelova², Sergey Dikalov³, Eva Nozik-Grayck¹

¹Cardiovascular Pulmonary Research Laboratories and Pediatric Critical Care Medicine, Department of Pediatrics, University of Colorado Anschutz Medical Campus, ²Bruker BioSpin Corp, ³Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center

JoVE 58461

A Fluorogenic Peptide Cleavage Assay to Screen for Proteolytic Activity: Applications for coronavirus spike protein activation

Javier A. Jaimes*^1,2, Jean K. Millet*^1,3, Monty E. Goldstein^1,4, Gary R. Whittaker¹, Marco R. Straus¹

¹Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, ²Department of Microbiology, College of Agricultural and Life Sciences, Cornell University, ³Virologie et Immunologie Moléculaires, Domaine de Vilvert, INRA, ⁴Department of Cell Biology and Molecular Genetics, University of Maryland

JoVE 58892

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