JoVE Journal

Biochemistry

JoVE Biochemistry publishes experimental approaches to investigate the structure, function, and interactions of biological molecules.

1 2 3 4 5 › 112 VIDEO ARTICLES

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Huijuan You*¹, Shimin Le*^2,3, Hu Chen^2,4, Linyan Qin¹, Jie Yan^2,4,5

¹School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, ²Mechanobiology Institute, National University of Singapore, ³Department of Physics, National University of Singapore, ⁴Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics, Xiamen University, ⁵Center for Bioimaging Sciences, National University of Singapore

JoVE 56328

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Antoine Loquet¹, James Tolchard¹, Melanie Berbon¹, Denis Martinez¹, Birgit Habenstein¹

¹Institute of Chemistry, Biology of Membranes, Nanoobjects, UMR5248 CNRS, Université de Bordeaux

JoVE 55779

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Pan Liu¹, Tomokazu Souma¹, Andrew Zu-Sern Wei¹, Xueying Xie³, Xunrong Luo², Jing Jin¹

¹Division of Nephrology and Hypertension, and the Center for Kidney Research and Therapeutics at the Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, ²Surgery-Organ Transplantation, Northwestern University Feinberg School of Medicine, ³School of Biological Sciences and Medical Engineering, Southeast University

JoVE 56278

Simple Elimination of Background Fluorescence in Formalin-Fixed Human Brain Tissue for Immunofluorescence Microscopy

Yulong Sun¹, Philbert Ip², Avijit Chakrabartty^1,2

¹Department of Medical Biophysics, University of Toronto, ²Department of Biochemistry, University of Toronto

JoVE 56188

In Vitro Polymerization of F-actin on Early Endosomes

Olivia Muriel^1,2, Cameron Christopher Scott¹, Jorge Larios¹, Vicent Mercier¹, Jean Gruenberg¹

¹Department of Biochemistry, University of Geneva, ²Department of Fundamental Microbiology, University of Lausanne

JoVE 55829

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Thomas Søndergaard Stenum¹, Michael A. Sørensen¹, Sine Lo Svenningsen¹

¹Department of Biology, University of Copenhagen

JoVE 56212

Quantitative Immunofluorescence to Measure Global Localized Translation

Jonathan Bergeman¹, Marc-Étienne Huot^1,2

¹Centre de Recherche sur le Cancer de l’Université Laval, Faculté de Médecine, Département de Biologie moléculaire, biochimie médicale et pathologie, Université Laval, ²CRCHU de Québec: L’Hôtel-Dieu de Québec

JoVE 55909

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Mo Zhou¹, Mark R. Philips¹

¹Langone Medical Center, New York University

JoVE 56037

Easy Manipulation of Architectures in Protein-based Hydrogels for Cell Culture Applications

Nicholas Bodenberger¹, Dennis Kubiczek¹, Frank Rosenau¹

¹Center for Peptide Pharmaceuticals, Faculty of Natural Science, Ulm University

JoVE 55813

Analysis of Histone Antibody Specificity with Peptide Microarrays

Evan M. Cornett¹, Bradley M. Dickson¹, Scott B. Rothbart¹

¹Center for Epigenetics, Van Andel Research Institute

JoVE 55912

Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

Megan E. Dowdle¹, Susanne Blaser Imboden¹, Sookhee Park¹, Sean P. Ryder², Michael D. Sheets¹

¹Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, ²Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School

JoVE 56031

mRNA Interactome Capture from Plant Protoplasts

Zhicheng Zhang¹, Kurt Boonen¹, Meixia Li¹, Koen Geuten¹

¹Department of Biology, KU Leuven

JoVE 56011

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Prateek Tripathi¹, José L. Pruneda-Paz², Steve A. Kay¹

¹Department of Neurology, University of Southern California, ²Section of Cell and Developmental Biology, University of California San Diego

JoVE 56080

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells

Thomas P. Karbanowicz¹, Ala Lew-Tabor^1,2, Manuel Rodriguez Valle¹

¹Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, ²Centre for Comparative Genomics, Murdoch University

JoVE 55747

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Marion Boudes*¹, Damià Garriga*¹, Fasséli Coulibaly¹

¹Infection and Immunity Program, Monash Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University

JoVE 55793

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

Padmamalini Baskaran¹, Baskaran Thyagarajan¹

¹School of Pharmacy, University of Wyoming

JoVE 55625

Determination of the Glycogen Content in Cyanobacteria

Alice De Porcellinis¹, Niels-Ulrik Frigaard², Yumiko Sakuragi³

¹Carlsberg Research Laboratory, ²Department of Biology, University of Copenhagen, ³Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, University of Copenhagen

JoVE 56068

Investigating Protein Sequence-structure-dynamics Relationships with Bio3D-web

Shashank Jariwala*¹, Lars Skjærven*², Xin-Qiu Yao¹, Barry J. Grant¹

¹Department of Computational Medicine and Bioinformatics, University of Michigan Medical School, ²Department of Biomedicine, University of Bergen

JoVE 55640

A Filtration-based Method of Preparing High-quality Nuclei from Cross-linked Skeletal Muscle for Chromatin Immunoprecipitation

Kazunari Nohara¹, Zheng Chen¹, Seung-Hee Yoo¹

¹Department of Biochemistry and Molecular Biology, University of Texas Health Science Center at Houston

JoVE 56013

Protein-tRNA Agarose Gel Retardation Assays for the Analysis of the N⁶-threonylcarbamoyladenosine TcdA Function

Francisco J. Fernández^1,2,3, Sara Gómez¹, Sergio Navas-Yuste¹, Miguel López-Estepa¹, M. Cristina Vega¹

¹Department of Physical Chemical Biology, Center for Biological Research (CIB-CSIC), Spanish National Research Council (CSIC), ²Department of Immunology, Complutense University School of Medicine, ³Abvance Biotech srl

JoVE 55638

Optimized Protocol for the Extraction of Proteins from the Human Mitral Valve

Cristina Banfi¹, Anna Guarino², Maura Brioschi¹, Stefania Ghilardi¹, Valeria Mastrullo¹, Elena Tremoli¹, Gianluca Polvani^2,3,4

¹Centro Cardiologico Monzino IRCCS, ²Cardiovascular Tissue Bank of Milan, Centro Cardiologico Monzino IRCCS, ³Department of Clinical Sciences and Community Health, Cardiovascular Section, University of Milan, ⁴Department of Cardiovascular Disease, Development and Innovation Cardiac Surgery Unit, Centro Cardiologico Monzino IRCCS

JoVE 55762

Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall

Xun Zhang¹, Sheng Chen¹, Feng Xu¹

¹Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University

JoVE 55910

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Mahnoush Bahjat¹, Timon A. Bloedjes¹, Amélie van der Veen¹, Guus de Wilde¹, Chiel Maas¹, Jeroen E. J. Guikema¹

¹Department of Pathology, Academic Medical Center, University of Amsterdam, Lymphoma and Myeloma Center Amsterdam (LYMMCARE)

JoVE 55703

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Chirag Vasavda*¹, Nicholas W. Zaccor*¹, Paul C. Scherer¹, Charlotte J. Sumner^1,2, Solomon H. Snyder^1,3,4

¹The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, ²Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, ³Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, ⁴Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine

JoVE 55561

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