JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

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Fixed Target Serial Data Collection at Diamond Light Source

Sam Horrell¹, Danny Axford¹, Nicholas E. Devenish¹, Ali Ebrahim¹, Michael A. Hough², Darren A. Sherrell^1,3, Selina L. S. Storm^1,4, Ivo Tews⁵, Jonathan A. R. Worrall², Robin L. Owen¹

¹Diamond Light Source, Harwell Science and Innovation Campus, ²School of Life Sciences, University of Essex, ³X-ray Science Division, Argonne National Laboratory, ⁴European Molecular Biology Laboratory, Hamburg Outstation c/o DESY, ⁵Biological Sciences, Institute for Life Sciences, University of Southampton

JoVE 62200

Plant Sample Preparation for Nucleoside/Nucleotide Content Measurement with An HPLC-MS/MS

Changhua Zhu*¹, Xiaoye Liu*², Wenlei Wang¹, Xiaoguang Chen³, Shangyu Gao¹, Meng Qian¹, Na Yang¹, Yifeng Xu¹, Mingjia Chen¹

¹College of Life Sciences, Nanjing Agricultural University, ²Department of Criminal Science and Technology, Nanjing Forest Police College, ³Department of Molecular Nutrition and Biochemistry of Plants, Institute of Plant Nutrition, Leibniz University Hannover

JoVE 61956

Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens

Alexander C. Y. Foo¹, Peter M. Thompson¹, Geoffrey A. Mueller¹

¹Nuclear Magnetic Resonance Group, National Institute of Environmental Health Sciences

JoVE 61780

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Noémie Mercier¹, Karine Prévost², Eric Massé², Pascale Romby¹, Isabelle Caldelari¹, David Lalaouna¹

¹Université de Strasbourg, CNRS, ²Department of Biochemistry, Université de Sherbrooke

JoVE 61731

Rapid Determination of Antibody-Antigen Affinity by Mass Photometry

Di Wu¹, Grzegorz Piszczek¹

¹Biophysics Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health

JoVE 61784

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Candice M. Etson^1,2, Petar Todorov¹, Nooshin Shatery Nejad², Nirmala Shrestha², David R. Walt^1,3

¹Department of Chemistry, Tufts University, ²Department of Physics, Wesleyan University, ³Wyss Institute at Harvard University

JoVE 62112

Myosin-Specific Adaptations of In vitro Fluorescence Microscopy-Based Motility Assays

Ananya Tripathi¹, Charles Bond^1,2, James R. Sellers¹, Neil Billington¹, Yasuharu Takagi¹

¹Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, ²Department of Physiology, Perelman School of Medicine, University of Pennsylvania

JoVE 62180

Methods Collection

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

John H. Beale¹, May E. Marsh¹

¹Swiss Light Source, Paul Scherrer Institut

JoVE 61896

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Sean Rogers¹, W. Mike Henne¹

¹Department of Cell Biology, University of Texas Southwestern Medical Center

JoVE 62201

Methods Collection

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Manabu Niimi¹, Haizhao Yan¹, Yajie Chen¹, Yao Wang², Jianglin Fan^1,2

¹Department of Molecular Pathology, Interdisciplinary Graduate School of Medicine, University of Yamanashi, ²School of Biotechnology and Health Sciences, Wuyi University

JoVE 61790

Methods Collection

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Mark D Tully*¹, Nicolas Tarbouriech², Robert P Rambo³, Stephanie Hutin*⁴

¹European Synchrotron Radiation Facility Structural Biology Group, Structural Biology Group, ²Institut de Biologie Structurale, University Grenoble Alpes, CEA, CNRS, ³Diamond Light Source, ⁴Laboratoire de Physiologie Cellulaire and Végétale, Université Grenoble Alpes/CNRS/CEA/INRA/BIG

JoVE 61578

Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs

Mégane Bregeon¹, Nicolas Guyot¹, Sophie Réhault-Godbert¹

¹Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Université de Tours, Biologie des Oiseaux et Aviculture, 37380, Nouzilly, France

JoVE 61739

Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening

Jia Quyen Truong*¹, Stephanie Nguyen*^1,2, John B. Bruning^1,2, Keith E. Shearwin¹

¹School of Biological Sciences, The University of Adelaide, ²Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide

JoVE 61894

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Karamat Mohammad¹, Heng Jiang², Vladimir I. Titorenko¹

¹Department of Biology, Concordia University, ²Centre for Biological Applications of Mass Spectrometry, Concordia University

JoVE 62061

Preparation of Site-Specific Cytotoxic Protein Conjugates via Maleimide-thiol Chemistry and Sortase A-Mediated Ligation

Mateusz Adam Krzyscik^1,2, Aleksandra Sokolowska-Wedzina¹, Karolina Jendryczko¹, Marta Pozniak¹, Daria Nawrocka¹, Natalia Porebska¹, Malgorzata Zakrzewska¹, Jacek Otlewski¹, Anna Szlachcic¹, Lukasz Opalinski¹

¹Department of Protein Engineering, Faculty of Biotechnology, University of Wroclaw, ²Department of Protein Biotechnology, Faculty of Biotechnology, University of Wroclaw

JoVE 61918

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli

Chesley Marie Rowlett¹, Wenshe Ray Liu¹

¹Department of Chemistry, Texas A&M University

JoVE 62113

Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide

Karidia Konate¹, Quentin Seisel¹, Eric Vivès¹, Prisca Boisguérin¹, Sébastien Deshayes¹

¹PHYMEDEXP, INSERM U1046 - CNRS UMR 9214 - University of Montpellier

JoVE 62028

Methods Collection

Complementation of Splicing Activity by a Galectin-3 - U1 snRNP Complex on Beads

Patricia G. Voss¹, Kevin C. Haudek¹, Ronald J. Patterson², John L. Wang¹

¹Department of Biochemistry and Molecular Biology, Michigan State University, ²Department of Microbiology and Molecular Genetics, Michigan State University

JoVE 61990

Methods Collection

Co-translational Insertion of Membrane Proteins into Preformed Nanodiscs

Roman Levin¹, Zoe Koeck¹, Volker Dötsch¹, Frank Bernhard¹

¹Institute of Biophysical Chemistry, Goethe University Frankfurt

JoVE 61844

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Sebastien Mailfert*¹, Karolina Wojtowicz*², Sophie Brustlein*¹, Ewa Blaszczak³, Nicolas Bertaux⁴, Marcin Łukaszewicz², Didier Marguet¹, Tomasz Trombik²

¹CNRS, Inserm, Centre d'Immunologie de Marseille-Luminy, Aix Marseille Univ, ²Faculty of Biotechnology, University of Wroclaw, ³Faculty of Biological Sciences, University of Wroclaw, ⁴CNRS, Centrale Marseille, Institut Fresnel, Aix Marseille Univ

JoVE 61823

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Felipe A. Carvalho¹, Moises R. A. Barros¹, Thierry P. F. Girotto¹, M. Griselda Perona¹, Carla C. Oliveira¹

¹Department of Biochemistry, Institute of Chemistry, University of Sao Paulo

JoVE 61994

Methods Collection

Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells

Kendall Martin¹, Tong Zhang¹, Pengfei Zhang², William B. Chrisler¹, Fillmore L. Thomas³, Fen Liu⁴, Tao Liu¹, Wei-Jun Qian¹, Richard D. Smith¹, Tujin Shi¹

¹Biological Sciences Division, Pacific Northwest National Laboratory, ²NHC Key Laboratory of Cancer Proteomics, Department of Oncology, Xiangya Hospital, Central South University, ³Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, ⁴Department of Critical Care Medicine, The First Affiliated Hospital of Nanchang University