JoVE Journal

Biochemistry

JoVE Biochemistry includes methods and experimental approaches to study the structure, function, and interactions of biological molecules.

1 2 3 4 5…8 › 172 VIDEO ARTICLES

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Kang-Yi Su*^1,2, Steven D. Goodman*³, Hung-Ming Lai¹, Rong-Syuan Yen¹, Wei-Yao Hu¹, Wern-Cherng Cheng², Liang-In Lin^1,2, Ya-Chien Yang^1,2, Woei-Horng Fang^1,2

¹Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, ²Department of Laboratory Medicine, National Taiwan University Hospital, ³Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Rosi Fassler*¹, Nufar Edinger*¹, Oded Rimon¹, Dana Reichmann¹

¹Department of Biological Chemistry, The Alexander Silberman Institute of Life Sciences, Safra Campus Givat Ram, The Hebrew University of Jerusalem

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Tabitha A. Peterson¹, Mark A. Stamnes¹, Robert C. Piper¹

¹Molecular Physiology and Biophysics, University of Iowa

An Alternative Culture Method to Maintain Genomic Hypomethylation of Mouse Embryonic Stem Cells Using MEK Inhibitor PD0325901 and Vitamin C

Cuiping Li¹, Weiyi Lai¹, Hailin Wang¹

¹State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences

The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Hiroyuki Yamamoto¹, Yoshikazu Sawaguchi², Michio Kimura¹

¹Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University, ²Department of Clinical Pharmaceutics, Nihon Pharmaceutical University

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Huang Chunhui^1,2, Xu Dilin¹, Zhang Ke¹, Shentu Jieyi², Yan Sicheng¹, Wu Dapeng³, Wang Qinwen¹, Cui Wei^1,2

¹Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, ²Li Dak Sum Yip Yio Chin Kenneth Li Marine Biopharmaceutical Research Center, Ningbo University, ³School of Materials Science and Chemical Engineering, Ningbo University

Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Joseph A. Ross¹, Nehal Thakor^1,2,3

¹Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, ²Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN), University of Lethbridge, ³Arnie Charbonneau Cancer Institute, University of Calgary

Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species

Kotaro Hama¹, Yuko Fujiwara¹, Kazuaki Yokoyama¹

¹Faculty of Pharmaceutical Sciences, Teikyo University

Measuring Trans-Plasma Membrane Electron Transport by C2C12 Myotubes

Shannon C. Kelly*¹, Amanda M. Eccardt*¹, Jonathan S. Fisher¹

¹Department of Biology, Saint Louis University

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Heying Cui*¹, Kyle M. Loftus*¹, Crystal R. Noell¹, Sozanne R. Solmaz¹

¹Department of Chemistry, State University of New York at Binghamton

Methods Collection

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Joshua T. Maxwell¹, Chin-Hsien Tsai¹, Tahmina A. Mohiuddin², Jennifer Q. Kwong¹

¹Department of Pediatrics, Emory University School of Medicine, ²Emory College of Arts and Sciences, Emory University

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Sarah Hanna-Addams¹, Zhigao Wang¹

¹Department of Molecular Biology, UT Southwestern Medical Center

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Isabel M. Schopp^1,2, Julien Béthune^1,2

¹Cluster of excellence CellNetworks, Heidelberg University, ²Heidelberg University Biochemistry Center (BZH)

Fatty Acid ¹³C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Ralph Menzel¹, Rainer Nehring¹, Dilara Simsek¹, Liliane Ruess¹

¹Ecology, Institute of Biology, Humboldt-Universität zu Berlin

Electrochemical Detection of Deuterium Kinetic Isotope Effect on Extracellular Electron Transport in Shewanella oneidensis MR-1

Yoshihide Tokunou¹, Kazuhito Hashimoto², Akihiro Okamoto²

¹Department of Applied Chemistry, University of Tokyo, ²Global Research Center for Environment and Energy based on Nanomaterials Science, National Institute for Materials Science

Protein Digestion, Ultrafiltration, and Size Exclusion Chromatography to Optimize the Isolation of Exosomes from Human Blood Plasma and Serum

Gustavo Diaz¹, Chandler Bridges¹, Megan Lucas¹, Yong Cheng², Jeff S. Schorey², Karen M. Dobos¹, Nicole A. Kruh-Garcia¹

¹Department of Microbiology, Immunology and Pathology, Colorado State University, ²Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Zemfira N. Karamysheva², Elena B. Tikhonova¹, Petar N. Grozdanov¹, James C. Huffman^2,3, Kristen R. Baca^1,3, Alexander Karamyshev¹, R. Brian Denison¹, Clinton C. MacDonald¹, Kai Zhang², Andrey L. Karamyshev¹

¹Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, ²Department of Biological Sciences, Texas Tech University, ³CISER (Center for the Integration of STEM Education & Research), Texas Tech University

Measurement of Mitochondrial Oxygen Consumption in Permeabilized Fibers of Drosophila Using Minimal Amounts of Tissue

Chloé J Simard*¹, Guillaume Pelletier*¹, Luc H Boudreau¹, Etienne Hebert-Chatelain², Nicolas Pichaud¹

¹Département de chimie et biochimie, Université de Moncton, ²Département de biologie, Université de Moncton

Detergent-free Ultrafast Reconstitution of Membrane Proteins into Lipid Bilayers Using Fusogenic Complementary-charged Proteoliposomes.

Mikhail A. Galkin¹, Aidan N. Russell², Steven B. Vik³, Richard M. Berry², Robert R. Ishmukhametov²

¹First Pavlov State Medical University, ²Clarendon Laboratory, Department of Physics, Oxford University, ³Department of Biological Sciences, Southern Methodist University

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

Lucero Romero-Aguilar*^1,2, Mónica Montero-Lomeli*³, Juan Pablo Pardo², Guadalupe Guerra- Sánchez¹

¹Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, ²Facultad de Medicina, Universidad Nacional Autónoma de México, ³Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro

Formation of Covalent DNA Adducts by Enzymatically Activated Carcinogens and Drugs In Vitro and Their Determination by ³²P-postlabeling

Marie Stiborova¹

¹Department of Biochemistry, Charles University

A Simple Method for High Throughput Chemical Screening in Caenorhabditis Elegans

Mark Lucanic¹, Theo Garrett¹, Matthew S. Gill², Gordon J. Lithgow¹

¹The Buck Institute for Research on Aging, ²The Scripps Research Institute Center on Aging

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Ozlem Yaren¹, Barry W. Alto², Kevin M. Bradley³, Patricia Moussatche³, Lyudmyla Glushakova³, Steven A. Benner^1,3

¹Foundation for Applied Molecular Evolution (FfAME), ²Florida Medical Entomology Laboratory, University of Florida, ³Firebird Biomolecular Sciences LLC

Regeneration of Arrayed Gold Microelectrodes Equipped for a Real-Time Cell Analyzer

Zhihui Xu*¹, Yiyan Song*¹, Huijun Jiang², Yan Kong³, Xiaoming Li³, Jin Chen¹, Yuan Wu⁴

¹School of Public Health, Nanjing Medical University, ²School of Pharmacy, Nanjing Medical University, ³State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering, Nanjing Tech University, ⁴Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University

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