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Adaptive Immunity: Protection from an infectious disease agent that is mediated by B- and T- Lymphocytes following exposure to specific antigen, and characterized by Immunologic memory. It can result from either previous infection with that agent or vaccination (Immunity, Active), or transfer of antibody or lymphocytes from an immune donor (Immunization, Passive).

An In Vivo Mouse Model to Measure Naïve CD4 T Cell Activation, Proliferation and Th1 Differentiation Induced by Bone Marrow-derived Dendritic Cells

1LamImSys Lab, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), 2LamImSys Lab, Instituto de Investigación Sanitaria Hospital 12 de Octubre (imas12), 3CIBER de Enfermedades Cardiovasculares

JoVE 58118


 Immunology and Infection

CRISPR Guide RNA Cloning for Mammalian Systems

1Wyss Institute for Biologically Inspired Engineering, Harvard University, 2Department of Genetics, Harvard Medical School, 3Department of Pathology, Massachusetts General Hospital, 4Institute for Medical Engineering & Science, Massachusetts Institute of Technology, 5Synthetic Biology Center, Massachusetts Institute of Technology, 6Department of Biological Engineering, Massachusetts Institute of Technology, 7Broad Institute

JoVE 57998


 Genetics

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

1Section of Pediatrics Infectious Diseases and Allergy, Department of Medicine, Imperial College London, 2Department of Pharmacy, National University of Singapore, 3MRC Center for Molecular Bacteriology and Infection, Department of Medicine, Imperial College London

Video Coming Soon

JoVE 59703


 JoVE In-Press

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

1Department of Endocrinology & Metabolism Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 2Berlin Institute of Health (BIH), 3Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 4DZHK (German Centre for Cardiovascular Research), 5Institute of Medical Immunology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, 6Julius Wolff Institute (JWI) and Center for Musculoskeletal Surgery, Charité - Universitätsmedizin Berlin, Berlin, Germany, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin

Video Coming Soon

JoVE 59470


 JoVE In-Press

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

1Surgery Branch, National Cancer Institute, NIH, 2Center for Cell-Based Therapy, National Cancer Institute, NIH, 3Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH

Video Coming Soon

JoVE 58672


 JoVE In-Press

Quantifying Leukocyte Egress via Lymphatic Vessels from Murine Skin and Tumors

1Department of Cell, Developmental, & Cancer Biology, Oregon Health and Science University, 2Department of Molecular Microbiology & Immunology, Oregon Health and Science University, 3Department of Dermatology, Oregon Health and Science University, 4Knight Cancer Institute, Oregon Health and Science University

JoVE 58704


 Biology

A Suction Blister Protocol to Study Human T-cell Recall Responses In Vivo

1Department of Infectious Disease Immunology, Center for Vaccine Research, Statens Serum Institut, 2Department of Infectious Diseases, Hvidovre Hospital, 3Institute of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, 4Division of Infection and Immunity, University College London

JoVE 57554


 Immunology and Infection

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

1Institute for Lung Research, Universities of Giessen and Marburg Lung Center, Philipps-University Marburg, 2German Center for Lung Research, 3Department of Medicine, Pulmonary and Critical Care Medicine, University Medical Center Giessen and Marburg

JoVE 55146


 Immunology and Infection

Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation

1Centre de Recherche en Cancérologie et Immunologie Nantes Angers (CRCINA), Institut national de la santé et de la recherche médicale (INSERM), Université d'Angers, Université de Nantes, Nantes, France, 2Service de chirurgie pédiatrique, Centre hospitalier universitaire (CHU) de Nantes, Nantes, France, 3Unité de Formation et Recherche (UFR) des Sciences Pharmaceutiques et Biologiques, Université de Nantes, Nantes, France

JoVE 58291


 Cancer Research

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

1Neurogenetics Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Section on Nervous System Development and Plasticity, The Eunice Kennedy Shriver National Institute of Child and Human Development, National Institutes of Health, 3Department of Molecular Biosciences, Rice Institute for Biomedical Research, Northwestern University, 4Department of Physiology, Anatomy and Genetics, University of Oxford

JoVE 55724


 Genetics

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