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Diabetes Mellitus: A heterogeneous group of disorders characterized by Hyperglycemia and Glucose intolerance.

Zebrafish Maintenance and Husbandry

JoVE 5152

The zebrafish (Danio rerio) is a powerful vertebrate model system for studying development, modeling disease, and screening for novel therapeutics. Due to their small size, large numbers of zebrafish can be housed in the laboratory at low cost. Although zebrafish are relatively easy to maintain, special consideration must be given to both diet and water quality to in order to optimize fish health and reproductive success. This video will provide an overview of zebrafish husbandry and maintenance in the lab. After a brief review of the natural zebrafish habitat, techniques essential to recreating this environment in the lab will be discussed, including key elements of fish facility water recirculation systems and the preparation of brine shrimp as part of the zebrafish diet. Additionally, the presentation will include information on how specific zebrafish strains are tracked in a laboratory setting, with specific reference to the collection of tail fin samples for DNA extraction and genotyping. Finally, experimental modifications of the zebrafish environment will be discussed as a means to further our understanding of these fish, and in turn, ourselves.


 Biology II

Observation and Inspection

JoVE 10119

Source: Jaideep S. Talwalkar, MD, Internal Medicine and Pediatrics, Yale School of Medicine, New Haven, CT

Observation and inspection is fundamental to physical examination and begins at the first point of contact with a patient. While observation and inspection are often used interchangeably, observation is a general term that refers to the careful use of one's senses to gain information. Inspection is an act limited to what one can observe visually, and when referring to physical examination, typically refers to findings on the surface of the body, rather than to behaviors. Skilled clinicians utilize all of their senses to assist with gaining an understanding of their patients, relying on vision, touch (percussion and palpation), and hearing (percussion and auscultation) primarily. Smell can also provide important diagnostic information during the patient encounter (e.g., personal hygiene, substance use, or metabolic diseases). Fortunately the sense of taste is largely a historical relic in medicine, though it is interesting to note that diabetes mellitus was diagnosed for many centuries by the sweet taste of the urine. Through experience, clinicians develop an important sixth sense - the gut instinct - that can only be gained through deliberate practice of clinical skills on thousands of pati


 Physical Examinations I

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro

1Department of Cellular and Integrative Physiology, Indiana University School of Medicine, 2Herman B Wells Center for Pediatric Research, Indiana University School of Medicine, 3Indiana Center for Biological Microscopy, Indiana University School of Medicine, 4Department of Medicine, Indiana University School of Medicine, 5Department of Pediatrics, Indiana University School of Medicine, 6Department of Microbiology and Immunology, Indiana University School of Medicine, 7Indiana University Simon Cancer Center, Indiana University School of Medicine

JoVE 57044


 Developmental Biology

Human Brown Adipose Tissue Depots Automatically Segmented by Positron Emission Tomography/Computed Tomography and Registered Magnetic Resonance Images

1Chemical and Physical Biology Program, Vanderbilt University, 2Department of Physical Medicine and Rehabilitation, Vanderbilt University School of Medicine, 3Radiology & Radiological Sciences, Vanderbilt University Medical Center, 4Department of Pharmacology, Vanderbilt University

JoVE 52415


 Medicine

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Phase Imaging and Machine Learning

1Department of Physics, University of Cambridge, 2Department of Physics, Korea Advanced Institute of Science and Technology, 3KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology, 4Tomocube, Inc., 5Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, 6Department of Biological Sciences, Korea Advanced Institute of Science and Technology

Video Coming Soon

JoVE 58305


 JoVE In-Press

Rat Model of Adhesive Capsulitis of the Shoulder

1Center for Advanced Orthopaedic Studies, Beth Israel Deaconess Medical Center and Harvard Medical School, 2Carl J. Shapiro Department of Orthopaedic Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, 3Departments of Biomedical Engineering, Chemistry, and Medicine, Boston University, 4Department of Orthopaedic Surgery, Yerevan State Medical University

JoVE 58335


 Bioengineering

Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

1Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 2Division of Endocrinology, Cincinnati Children's Hospital Medical Center, 3Division of Developmental Biology, Cincinnati Children's Hospital Medical Center, 4Department of Pediatrics, College of Medicine, University of Cincinnati

JoVE 58323


 Medicine

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

1Graduate Institute of Medical Sciences, National Defense Medical Center, 2Department and Graduate Institute of Biology and Anatomy, National Defense Medical Center, 3Department of Gerontological Health Care, National Taipei University of Nursing and Health Sciences, 4Division of Rheumatology/Immunology/Allergy, Department of Internal Medicine, Tri-Service General Hospital, National Defense Medical Center, 5Department of Plastic and Reconstructive Surgery, Tri-Service General Hospital, 6Department of Nursing, Yuanpei University, 7Department of Physiology and Biophysics; Graduate Institute of Physiology, National Defense Medical Center, 8Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center

Video Coming Soon

JoVE 57942


 JoVE In-Press

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

15th Medical Department, Medical Faculty Mannheim, Heidelberg University, 2Department of Internal Medicine, Heidelberg University, 3German Center for Diabetes Research (DZD), 4European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University

JoVE 58117


 Biochemistry

Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease

1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine, University of Freiburg, 2Department of Medicine, Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center, University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital, University of Cologne

JoVE 57950


 Medicine

A Method for Islet Transplantation to the Omentum in Mouse

1Shenzhen Xenotransplantation Medical Engineering Research and Development Center, Institute of Translational Medicine, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, 2Institute for Cellular Therapeutics, Allegheny-Singer Research Institute, 3Xenotransplantation Program/Department of Surgery, The University of Alabama at Birmingham, 4College of Life Science and Oceanography, Shenzhen University, 5School of Pharmcy, Wenzhou Medical College

Video Coming Soon

JoVE 57160


 JoVE In-Press

Fat-Water Phantoms for Magnetic Resonance Imaging Validation: A Flexible and Scalable Protocol

1Vanderbilt University Institute of Imaging Science (VUIIS), Vanderbilt University Medical Center, 2Department of Biomedical Informatics, Vanderbilt University Medical Center, 3Department of Physical Medicine and Rehabilitation, Vanderbilt University Medical Center, 4Department of Biomedical Sciences, Grand Valley State University, 5Department of Radiology & Radiological Sciences, Vanderbilt University Medical Center, 6Department of Biomedical Engineering, Vanderbilt University, 7Department of Molecular Physiology and Biophysics, Vanderbilt University

JoVE 57704


 Medicine

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