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Tumor Microenvironment: The milieu surrounding neoplasms consisting of cells, vessels, soluble factors, and molecules, that can influence and be influenced by, the neoplasm's growth.

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies

1Department of Cancer Biology, Dana-Farber Cancer Institute, 2Department of Pediatrics, Children's Hospital, 3Department of Biostatistics & Computational Biology, Dana-Farber Cancer Institute, 4Department of Developmental Biology and Cancer Research, Hebrew University of Jerusalem, 5Department of Neurosurgery, Children's Hospital, 6Center for Molecular Oncologic Pathology, Department of Medical Oncology, Dana-Farber Cancer Institute

JoVE 53304


 Medicine

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment

1In Vivo Multifunctional Magnetic Resonance center, Robert C. Byrd Health Sciences Center, West Virginia University, 2Department of Biochemistry, West Virginia University School of Medicine, 3Department of Microbiology, Immunology & Cell Biology, West Virginia University School of Medicine

JoVE 56624


 Cancer Research

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 2Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 3Intravital Imaging Program, Albert Einstein College of Medicine, 4Department of Surgery, Montefiore Medical Center, 5Department of Pathology, Montefiore Medical Center

Video Coming Soon

JoVE 59633


 JoVE In-Press

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

1PARCC-INSERM U970, Université Paris Descartes, 2INSERM U970, Université Paris Descartes, 3Equipe Labellisée Ligue Contre le Cancer, 4Department of Immunology, Hopital Européen Georges Pompidou, 5Department of Medical Urology, Hopital Européen Georges Pompidou, 6Department of Pathology, Hopital Européen Georges Pompidou, 7Université Paris Diderot Paris 7, 8Department of medical oncology, Hopital Européen Georges Pompidou

JoVE 56606


 Cancer Research

Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma

1Department of Laboratory Medicine, AnSteel Group General Hospital, 2Cancer Science Institute of Singapore, National University of Singapore, 3Department of Pathology, Yong Loo Lin School of Medicine, National University of Singapore, 4Department of Haematology-Oncology, National University Health System

JoVE 58711


 Immunology and Infection

Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

1The Centenary Institute, 2Sydney Medical School, University of Sydney, 3The University of Queensland Diamantina Institute, Translational Research Institute, The University of Queensland, 4Department of Dermatology, Royal Prince Alfred Hospital, 5Discipline of Dermatology, University of Sydney

JoVE 53486


 Medicine

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

1Royal (Dick) School of Veterinary Studies and Roslin Institute, University of Edinburgh, 2MRC Centre for Reproductive Health, University of Edinburgh, 3Edinburgh Phenotypic Assay Centre, University of Edinburgh, 4Cancer Research UK Edinburgh Centre, MRC Institute of Genetics, Molecular Medicine, University of Edinburgh

JoVE 58841


 Cancer Research

Automated Multiplex Immunofluorescence Panel for Immuno-oncology Studies on Formalin-fixed Carcinoma Tissue Specimens

1Laboratory of Pathology, MedImmune, 2Laboratory of Pathology, MedImmune, 3Akoya Biosciences Inc., 4Department of Translational Molecular Pathology, University of Texas - MD Anderson Cancer Center

JoVE 58390


 Cancer Research

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

1Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, 2Department of Obstetrics/Gynecology and Woman’s Health, Albert Einstein College of Medicine, 3Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, 4Gruss-Lipper Biophotonics Center Integrated Imaging Program, Albert Einstein College of Medicine, 5Medical Research Council Centre for Reproductive Health, Queen’s Medical Research Institute, University of Edinburgh

JoVE 54603


 Cancer Research

Bioprintable Alginate/Gelatin Hydrogel 3D In Vitro Model Systems Induce Cell Spheroid Formation

1Department of Mechanical Engineering, McGill University Montreal, 2Department of Bioengineering, McGill University Montreal, 3Department of Molecular Biology, Instituto Potosino de Investigación Científica y Tecnológica, A.C. (IPICyT), 4Department of Mining and Materials Engineering, McGill University Montreal, 5Department of Biomedical Engineering, McGill University Montreal

JoVE 57826


 Bioengineering

In Vivo Model for Testing Effect of Hypoxia on Tumor Metastasis

1Department of Biochemistry and Molecular & Cellular Biology, Georgetown University Medical Center, 2Department of Nursing, Georgetown University, School of Nursing and Health Studies, 3Department of Human Science, Georgetown University, School of Nursing and Health Studies, 4School of Medicine, Georgetown University Medical Center, 5Department of Pathology and Neuropathology, Medical University of Gdańsk, 6Department of Oncology, Georgetown University Medical Center, 7Department of Pathology, Georgetown University Medical Center

JoVE 54532


 Cancer Research

A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model

1Division of Gastroenterology, Hepatology, and Nutrition, Department of Internal Medicine, University of Utah, 2Huntsman Cancer Institute, University of Utah

JoVE 57182


 Cancer Research

An Oncogenic Hepatocyte-Induced Orthotopic Mouse Model of Hepatocellular Cancer Arising in the Setting of Hepatic Inflammation and Fibrosis.

1Department of Surgery, University of Missouri-Columbia, 2Ellis Fischel Cancer Center, University of Missouri-Columbia, 3Molecular Microbiology and Immunology, University of Missouri-Columbia

Video Coming Soon

JoVE 59368


 JoVE In-Press

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

1Department of Life Sciences, University of Trieste, 2Institute for Maternal and Child Health, IRCCS (Istituto di Ricovero e Cura a Carattere Scientifico) Burlo Garofolo, 33. Department of Medical, Surgical and Health Science, University of Trieste

Video Coming Soon

JoVE 58688


 JoVE In-Press

Immunohistochemical Staining of CD8 Protein in Tissue Sections from Patients with Tumors

1Department of Urology, the Seventh Affiliated Hospital, Sun Yat-sen University, 2Zhongshan School of Medicine, Sun Yat-sen University, 3Guanghua School of Stomatology, Sun Yat-sen University, 4Department of Pathology, the Seventh Affiliated Hospital, Sun Yat-sen University

Video Coming Soon

JoVE 57945


 JoVE In-Press

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes

1Department of Integrated Traditional Chinese and Western Medicine, West China Hospital of Sichuan University, 2Virginia G. Piper Biodesign Center for Personalized Diagnostics, The Biodesign Institute, Arizona State University, 3School of Biological and Health Systems Engineering, Arizona State University, 4Department of Electrical and Computer Engineering, North Dakota State University

Video Coming Soon

JoVE 59177


 JoVE In-Press

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