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Immunology and Infection

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JoVE Journal Immunology and Infection
Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Article DOI: 10.3791/3076-v • 12:50 min • August 9th, 2011
August 9th, 2011 •
1, 2, 2, 1
1St Vincent’s Institute, Department of Medicine, The University of Melbourne, 2Department of Microbiology and Immunology, The University of Melbourne

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Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.

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Bacterial Load Immune Responses Mice Listeria Monocytogenes Gram-positive Intracellular Pathogen Systemic Infection Spleen Liver CD8α+ Dendritic Cells Kupffer Cells Phagocytosed Bacterial Proteins Phagosome Cytosol Innate Immune Cells Monocytes Neutrophils NK Cells Dendritic Cells Bactericidal Mechanisms CD8+ T Cells Clearance Of Listeria Host Immune Responses Inbred Mouse Strains Genetic Studies
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