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Monitoring ER/SR Calcium Release with the Targeted Ca2+ Sensor CatchER+

Article DOI: 10.3791/55822-v • 12:30 min • May 19th, 2017
May 19th, 2017 •
1, 1, 1, 1
1Department of Chemistry, Center of Diagnostics and Therapeutics (CDT), Georgia State University

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We present protocols for the application of our targeted genetically-encoded calcium indicator (GECI) CatchER+ for monitoring rapid calcium transients in the endoplasmic/sarcoplasmic reticulum (ER/SR) of HEK293 and skeletal muscle C2C12 cells using real-time fluorescence microscopy. A protocol for the in situ Kd measurement and calibration is also discussed.

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ER/SR Calcium Release Targeted Ca2+ Sensor CatchER+ Experiment Protocols Culture And Transfection HEK293 Cells C2C12 Cells Calcium Transients Measurement Of NC2KD Basal Calcium Concentration Rapid Changes In ERSR Biosensor CatchER+ Fluorescence Response Real-time Kinetics Calcium Signaling Elements Calcium-related Diseases Abnormal Calcium Transients Organelles Dysfunctional Calcium Signaling Elements Cell Transfection Fluorescence Imaging Florence Reddish Post-doc Glass Microscope Slides Six Centimeter Dishes Reduced Serum Med
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