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Cancer Research

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JoVE Journal Cancer Research
Bruker CRISPR/Cas9 Gene redigering for å undersøke kreftfremkallende aktiviteten av muterte Calreticulin i cytokin avhengige blodkreft cellene
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Bruker CRISPR/Cas9 Gene redigering for å undersøke kreftfremkallende aktiviteten av muterte Calreticulin i cytokin avhengige blodkreft cellene

Article DOI: 10.3791/56726-v • 10:21 min • January 5th, 2018
January 5th, 2018 •
1, 1,2,3
1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Broad Institute, 3Dana-Farber Cancer Institute, Harvard Medical School

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Målrettet genet redigering med CRISPR/Cas9 har mye lettere forståelsen av biologiske funksjoner gener. Her bruker vi CRISPR/Cas9 metodikken å modell calreticulin mutasjoner i cytokin-avhengige blodkreft cellene for å studere aktiviteten deres kreftfremkallende.

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Kreftforskning problemet 131 CRISPR Cas9 gen redigering sgRNA calreticulin sekvensering reporter
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