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JoVE Journal Neuroscience
Visualisering af Motor Axon Navigation og kvantificering af Axon Arborization i mus embryoner ved hjælp af lys ark Fluorescens mikroskopi
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Visualisering af Motor Axon Navigation og kvantificering af Axon Arborization i mus embryoner ved hjælp af lys ark Fluorescens mikroskopi

Article DOI: 10.3791/57546-v • 08:56 min • May 11th, 2018
May 11th, 2018 •
*1,2, *2,3, 1,2
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences, National Defense Medical Center, 2Institute of Molecular Biology, Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture, National Taiwan University
* These authors contributed equally

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Her, beskriver vi en protokol til at visualisere motorneuron projektion og axon arborization i transgene Hb9::GFP mus embryoner. Efter immunfarvning for motoriske neuroner brugte vi lys ark Fluorescens mikroskopi billede embryoner til efterfølgende kvantitativ analyse. Denne protokol finder anvendelse på andre neuron navigation processer i centralnervesystemet.

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Neurovidenskab spørgsmålet 135 Motor neuron axon navigation axon bane axon arborization lys ark mikroskopi spinal cord IMARIS
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