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Visualisering av Motor Axon navigering och kvantifiering av Axon Arborization i musembryon som använder ljus ark fluorescensmikroskopi

Article DOI: 10.3791/57546-v • 08:56 min • May 11th, 2018
May 11th, 2018 •
*1,2, *2,3, 1,2
1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences, National Defense Medical Center, 2Institute of Molecular Biology, Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture, National Taiwan University
* These authors contributed equally

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Här beskriver vi ett protokoll för att visualisera motorneuronen projektion och axon arborization i transgena Hb9::GFP musembryon. Efter immunfärgning för motoriska nervceller använde vi ljus ark fluorescensmikroskopi bild embryon för efterföljande kvantitativ analys. Detta protokoll är tillämpligt till andra neuron navigering processer i det centrala nervsystemet.

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Neurovetenskap fråga 135 Motor neuron axon navigering axon bana axon arborization lätta blad mikroskopi spinal cord IMARIS
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