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Cancer Research

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En 3D spheroid modell som en mer fysiologisk system for kreft-Associated fibroblaster differensiering og Invasion in vitro studier

Article DOI: 10.3791/60122-v • 06:27 min • August 8th, 2019
August 8th, 2019 •
1,2, 1
1Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, 2Instituto de Medicina Molecular, Lisbon

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Målet med denne protokollen er å etablere en 3D in vitro-modell for å studere differensiering av kreft-assosiert fibroblaster (CAFs) i en svulst bulk-lignende miljø, som kan håndteres i ulike analyse systemer, for eksempel immunofluorescence, transcriptional analyse og livs celle avbildning.

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Kreftforskning spheroids CAF 3D-modell differensiering immunofluorescent farging qPCR flyt flowcytometri invasjon ekstracellulære matrise gel
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