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Differentiatie en karakterisering van neurale voorlopercellen en neuronen uit embryonale stamcellen van muizen

Article DOI: 10.3791/61446-v • 08:47 min • May 15th, 2020
May 15th, 2020 •
1, 1, 1, 1
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute

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We beschrijven de procedure voor de in vitro differentiatie van embryonale stamcellen van muizen in neuronale cellen met behulp van de hanging drop-methode. Verder voeren we een uitgebreide fenotypische analyse uit via RT-qPCR, immunofluorescentie, RNA-seq en flowcytometrie.

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Ontwikkelingsbiologie Nummer 159 Embryonale stamcellen van muizen embryoïde lichamen neurale voorlopercellen neuronen differentiatie hangende druppel E14
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