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JoVE Journal
Bioengineering

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寡核苷酸的化学三磷酸化
 
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寡核苷酸的化学三磷酸化

Article DOI: 10.3791/63877-v 13:19 min June 2nd, 2022
June 2nd, 2022

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Summary

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Click here for the English version.

寡核苷酸5′-三磷酸盐是基本生物学途径中无处不在的成分,并且在生物技术应用中的使用越来越多。在这里,我们描述了从标准自动合成技术制备的寡核苷酸开始,常规合成和纯化寡核苷酸5′-三磷酸的技术。

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生物工程,第184期,
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