/
/
Generating Controlled, Dynamic Chemical Landscapes to Study Microbial Behavior
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Institute of Environmental Engineering, Department of Civil, Environmental and Geomatic Engineering, 2School of Mathematics and Statistics,University of Melbourne, 3Institute of Marine Sciences,University of California, Santa Cruz, 4Faculty of Life and Environmental Sciences,University of Tsukuba, 5Microbiology Research Center for Sustainability,University of Tsukuba, 6Department of Ecology and Evolutionary Biology,Princeton University
Chapters
- 00:05Introduction
- 00:35Fabrication of the Microfluidic Device for the Single Chemical-pulse Experiment
- 02:02Fabrication of the 3D-printed Millifluidic Device for the Experiment with Multiple Pulses
- 05:28Multiple Chemical-pulse Experiment
- 07:11Results: Bacterial Responses to a Chemical Pulse and Swimming Statistics in the Absence of Chemical Gradients
- 08:50Conclusion
A protocol for the generation of dynamic chemical landscapes by photolysis within microfluidic and millifluidic setups is presented. This methodology is suitable to study diverse biological processes, including the motile behavior, nutrient uptake, or adaptation to chemicals of microorganisms, both at the single cell and population level.
