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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

DOI:
10.3791/50618-v

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15:41 min

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October 15, 2013

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, , ,
1Department of Chemistry and Applied Biosciences,ETH Zurich, Switzerland

Chapters

  • 00:05Title
  • 02:13Fabrication of the Microfluidic Chip
  • 05:31Chip Preparation for Enzymatic Assays
  • 07:15Additional Chip Preparation for Immunoassays
  • 10:05Cell Harvesting and Glucose 6-Phosphate Dehydrogenase Assay
  • 12:08Results: Enzymatic and Antibody Analysis of Single Cells
  • 15:08Conclusion

Summary

Automatic Translation

Automatic Translation

In this article we present a microfluidic chip for single cell analysis. It allows the quantification of intracellular proteins, enzymes, cofactors, and second messengers by means of fluorescent assays or immunoassays. 

Tags

Microfluidic ChipSingle-cell AnalysisIntracellular BiomoleculesQuantitative DeterminationCell IsolationCell LysisLysate RetentionHigh SensitivityArray FormatAnalysis Of ProteinsEnzymesCofactorsSecond Messengers

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