Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Kapitel 15
Was ist Gentechnik?
Genetic engineering is the process of modifying an organism’s DNA to introduce new, desirable traits. Many organisms, from bacteria to plants…
Antibiotische Selektion
Researchers use antibiotic resistance genes to identify bacteria that possess a plasmid containing their gene of interest. Antibiotic resistance…
Rekombinierte DNA
Scientists create recombinant DNA by combining DNA from different sources—often, other species—in the laboratory. DNA cloning allows…
Transgene Organismen
Transgenic organisms are genetically engineered to carry transgenes—genes from a different species—as part of their genome. The…
Erwachsene Stammzellen
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell…
Embryonale Stammzellen
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous…
Induzierte pluripotente Stammzellen
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific…
In-vitro-Mutagenese
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by…
DNA-Isolierung
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells,…
Die Gentherapie
Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a…
Reproduktives Klonen
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced…
CRISPR
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at…
Komplementäre DNA
Only genes that are transcribed into messenger RNA (mRNA) are active, or expressed. Scientists can, therefore, extract the mRNA from cells to study…
PCR
The polymerase chain reaction, or PCR, is a widely used technique for copying segments of DNA. Due to exponential amplification, PCR can produce…
 Genomik
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried…
Alternative Kulturen für menschlichen pluripotenten Stammzellen Produktion, Wartung und Genetic Analysis
Human pluripotent stem cells (hPSCs) hold great promise for regenerative medicine and biopharmaceutical applications. Currently, optimal culture and…
Gezielte Sequenzierung der nächsten Generation und Bioinformatik-Pipeline, um genetische Determinanten der konstitutionelle Krankheit zu bewerten
Next-generation sequencing (NGS) is quickly revolutionizing how research into the genetic determinants of constitutional disease is performed. The…
Die Multi-Organ Chip - Ein Mikrofluidik-Plattform für langfristige Multi-Gewebe Cokultur
The ever growing amount of new substances released onto the market and the limited predictability of current in vitro test systems has led to a high…
CRISPR Guide RNA Klonen für Säugetier-Systeme
The outlined protocol describes streamlined methods for the efficient and cost-effective generation of Cas9-associated guide RNAs. Two alternative…
CAPRRESI: Chimären Assembly durch Plasmid Recovery und Restriktion Enzym Site Insertion
Here, we present chimera assembly by plasmid recovery and restriction enzyme site insertion (CAPRRESI). CAPRRESI benefits from many strengths of the…
Synthese von Keratin-basierten Nanofaser für Biomedizinische Technik
Electrospinning, due to its versatility and potential for applications in various fields, is being frequently used to fabricate nanofibers.…
Protokolle für die Beurteilung der Radiofrequenz-Wechselwirkungen mit Gold-Nanopartikel und biologische Systeme für die Nicht-invasive Krebstherapie Hyperthermie
Cancer therapies which are less toxic and invasive than their existing counterparts are highly desirable. The use of RF electric-fields that…

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter