Materials
Name | Company | Catalog Number | Comments |
Stereotaxic apparatus | ASI Instruments | SAS-4100 | |
Dissecting microscope | World Precision Instruments, Inc. | PZMIV-BS | |
Scalpel handle | Fine Science Tools | 10003-12 | |
Scalpel Blades #15 | Fine Science Tools | 10015-00 | |
Microdrill | Fine Science Tools | 18000-17 | |
Suture needle | Fine Science Tools | 12050-03 | |
Spring scissors | Fine Science Tools | 16144-13 | |
Dumont #5 forceps | Fine Science Tools | 11252-50 | |
Hamilton syringe | Hamilton Co | 88400 | |
Gauze sponges | Office Depot | 674889 | |
Fluorochrome | Sigma-Aldrich | 39286 | |
Alcohol | Sigma-Aldrich | 459844 | 70% |
H2O2 | Sigma-Aldrich | H3410 | 3% |
References
- Yoles, E. GM1 reduces injury-induced metabolic deficits and degeneration in the rat optic nerve. Investigative ophthalmology & visual science. 33, 3586-3591 (1992).
- Fisher, J. Vaccination for neuroprotection in the mouse optic nerve: implications for optic neuropathies. J Neurosci. 21, 136-142 (2001).
- Levkovitch-Verbin, H. RGC death in mice after optic nerve crush injury: oxidative stress and neuroprotection. Investigative ophthalmology & visual science. 41, 4169-4174 (2000).
- Li, Y. VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats. The Journal of clinical investigation. 118, 913-923 (2008).
- Tang, Z. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation. The Journal of experimental medicine. 207, 867-880 (2010).
- Gustmann, S., Dunker, N. In vivo-like organotypic murine retinal wholemount culture. J Vis Exp. , (2010).
- Franklin, K., Paxinos, G. The Mouse Brain in Stereotaxic Coordinates. , Academic Press. San Diego. (1997).