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Medicine

Forbedring af apoptotiske og Autophagic Induktion af en hidtil ukendt syntetisk C-1 analog af 7-deoxypancratistatin i human bryst adenocarcinom og neuroblastomceller med tamoxifen

doi: 10.3791/3586 Published: May 30, 2012

Materials

Name Company Catalog Number Comments
SH-SY5Y cell line ATCC CRL-2266
Dulbecco’s Modified Eagles Medium F-12 HAM Sigma-Aldrich 51448C
Fetal bovine serum GIBCO, by Life Technologies 16000-044
MCF7 cell line ATCC HTB-22
RPMI-1640 medium Sigma-Aldrich R 0883
Apparently normal human fetal fibroblast cell line (NFF) Coriell Institute for Medical Research AG04431B
Dulbecco’s Modified Eagle’s Medium, High Glucose medium Thermo Fisher Scientific, Inc. SH30022.01
Tamoxifen citrate salt Sigma-Aldrich T9262
35 mm glass bottom culture dishes MatTek Corp. P35G-014-C
Leica DMI6000 B inverted microscope Leica Microsystems N/A
H–chst 33342 dye Molecular Probes, Life Technologies H3570
Leica DM IRB inverted fluorescence microscope Leica Microsystems N/A
Annexin V AlexaFluor-488 Invitrogen A13201
Trypan Blue solution Sigma-Aldrich T8154-20ML
Haemocytometer Fisher Scientific 267110
WST-1 reagent Roche Group 11644807001
Wallac Victor3 1420 Multilabel Counter PerkinElmer, Inc. 1420-011
Tetramethylrhodamine methyl ester (TMRM) GIBCO, by Life Technologies T-668
Glass tissue grinder Fisher Scientific K8885300-0002
BioRad protein assay Bio-Rad 500-0001Bottom of Form
Amplex Red Invitrogen A12222
Horseradish peroxidase (HRP) Sigma-Aldrich P8125
SpectraMax Gemini XPS Molecular Devices 3126666
Anti-LC3 antibody raised in rabbit Novus Biologicals NB100-2220
Anti-mouse HRP-conjugated secondary antibody Abcam ab6728
Anti-rabbit HRP-conjugated secondary antibody Abcam ab6802
Chemiluminescence peroxidase substrate Sigma-Aldrich CPS160
Monodansylcadaverine Sigma-Aldrich 30432

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References

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Forbedring af apoptotiske og Autophagic Induktion af en hidtil ukendt syntetisk C-1 analog af 7-deoxypancratistatin i human bryst adenocarcinom og neuroblastomceller med tamoxifen
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Cite this Article

Ma, D., Collins, J., Hudlicky, T., Pandey, S. Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen. J. Vis. Exp. (63), e3586, doi:10.3791/3586 (2012).More

Ma, D., Collins, J., Hudlicky, T., Pandey, S. Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen. J. Vis. Exp. (63), e3586, doi:10.3791/3586 (2012).

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