Materials
| Name | Company | Catalog Number | Comments |
| 1X PBS, Ca2+- and Mg2+-free | |||
| Hank's balanced salt solution (HBSS) with phenol red | Fisher Scientific | SH3001603 | |
| Sodium bicarbonate | Sigma-Aldrich | S6014 | |
| 1M HEPES in 0.85% NaCl | Lonza Inc. | 17-737E | |
| Fetal bovine serum (FBS) | Atlanta Biologicals | S11150H | Heat-inactivated |
| 0.5M EDTA (pH 8.0) | Cellgro | 46-034-CI | |
| Collagenase type VIII | Sigma-Aldrich | C2139 | |
| DNase I | Roche Group | 14785000 | Stock solution: 100mg/ml |
| LIVE/DEAD Fixable Aqua Dead Cell Stain Kit for 405 nm excitation | Invitrogen | L34957 | Use at 1:1000 |
| CD45-PerCP mAb (30F11) | BD Biosciences | 557235 | Use at 1:100 |
| CD103-PE mAb (M290) | BD Biosciences | 557495 | Use at 1:100 |
| FcγRIII/II mAb (2.4G2) | BD Biosciences | 553141 | Use at 1:200 |
| CD11c-APC mAb (N418) | eBioscience | 17-0114-82 | Use at 1:100 |
| MHC-II (I-Ab)-Alexa Fluor 700 mAb | eBioscience | 56-5321-82 | Use at 1:100 |
| CD11b-eFluor 450 mAb (M1/70) | eBioscience | 48-0112-82 | Use at 1:200 |
| F4/80-PE-Cy7 mAb (BM8) | eBioscience | 25-4801-82 | |
| CD11b microbeads | Miltenyi Biotec | 130-049-601 | |
| CD11c microbeads | Miltenyi Biotec | 130-052-001 | |
| 50 mL conical tubes | BD Biosciences | 352098 | |
| Single mesh wire strainer | Chefmate | ||
| Small weigh boat | Fisher Scientific | 08-732-116 | |
| 100 μm cell strainer | BD Biosciences | 352360 | |
| 40 μm cell strainer | BD Biosciences | 352340 | |
| 5 mL polystyrene round-bottom tubes | BD Biosciences | 352235 | Use at 1:100 |
| MaxQ 4450 benchtop orbital shaker | Thermo Fisher Scientific, Inc. | ||
| LS MACS column | Miltenyi Biotec | 130-042-401 | |
| LSR II | BD Biosciences | ||
| FACSAria II | BD Biosciences |
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