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Medicine

जीन डिलीवरी के अध्ययन के लिए एक orthotopic मूत्राशय कैंसर के मॉडल

doi: 10.3791/50181 Published: December 1, 2013

Materials

Name Company Catalog Number Comments

Name of reagent

Company

Catalog number

Comments

6-8 week old female mice

Jackson Laboratories

Strain Name: C57BL/6J

Stock Number: 000664

Trypsin*

MediaTech

MT25-053-CI

Obtained through Fisher

DMEM*

MediaTech

MT10-017-CV

Obtained through Fisher

FBS

Hyclone

SH30071.03

Heat-inactivated

T25 flasks*

Corning Costar

Corning No.:3056

Fisher: 07-200-63

Obtained through Fisher

MB49 cells

N/A

N/A

Obtained from Dr. Boehle (see reference11)

Puralube Vet Ointment*

Pharmaderm

Henry Schein Company

No.:036090-6050059

Fisher: NC9676869

Obtained through Fisher

Depilatory cream: Veet

local pharmacy

Lubricant:

K-Y Jelly

local pharmacy

Catheters*

Exel International

Exel International

No.:26751;

Fisher: 14-841-21

Obtained through Fisher

Isoflurane

Terrell

NDC 66794-011-25

Obtained though hospital pharmacy

1 ml slip tip TB syringes

Becton Dickinson

BD309659

Fisher:14-823-434

D-Luciferin

Gold Biotechnologies

L-123-1

Ad-CMV-Luc

VectorBiolabs

1000; Request large scale amplification and CsCl purification for in vivo use

Infectious agent that requires BSL2 containment

Steady-Glo Luciferase Assay System

Promega

E2510 (10 ml), E2520 (100 ml), or E2550 (10 x 100 ml)

*available through multiple vendors

EQUIPMENT

Anesthesia system

E-Z Systems, Euthanex Corporation

Anesthesia system: EZ7000

5-port mouse rebreathing device: EZ109

Obtained through Fisher

Xenogen IVIS 200

Caliper Life Sciences

http://www.caliperls.com/products/preclinical-imaging/ivis-imaging-system-200-series.htm

FLUOstar Optima

BMG Labtech

http://www.bmglabtech.com/products/microplate-reader/instruments.cfm?product_id=2

DOWNLOAD MATERIALS LIST

References

  1. Siegel, R., Naishadham, D., Jemal, A. Cancer statistics, 2012. CA Cancer. J. Clin. 62, (1), 10-29 (2012).
  2. Seager, C. M., Puzio-Kuter, A. M., et al. Intravesical delivery of rapamycin suppresses tumorigenesis in a mouse model of progressive bladder cancer. Cancer Prev. Res. 2, (12), 1008-1014 (2009).
  3. Chodak, G. W., Shing, Y., Borge, M., Judge, S. M., Klagsbrun, M. Presence of heparin binding growth factor in mouse bladder tumors and urine from mice with bladder cancer. Cancer Res. 46, (11), 5507-5510 (1986).
  4. De Boer, E. C., Teppema, J. S., Steerenberg, P. A., De Jong, W. H. Retrovirus type C in the mouse bladder carcinoma cell line MBT-2. J. Urol. 163, (6), 1999-2001 (2000).
  5. Lodillinsky, C., Rodriguez, V., et al. Novel invasive orthotopic bladder cancer model with high cathepsin B activity resembling human bladder cancer. J. Urol. 182, (2), 749-755 (2009).
  6. Jurczok, A., Fornara, P., Soling, A. Bioluminescence imaging to monitor bladder cancer cell adhesion in vivo: a new approach to optimize a syngeneic, orthotopic, murine bladder cancer model. BJU Int. 101, (1), 120-124 (2008).
  7. Brocks, C. P., Buttner, H., Bohle, A. Inhibition of tumor implantation by intravesical gemcitabine in a murine model of superficial bladder cancer. J. Urol. 174, (3), 1115-1118 (2005).
  8. Wu, Q., Esuvaranathan, K., Mahendran, R. Monitoring the response of orthotopic bladder tumors to granulocyte macrophage colony-stimulating factor therapy using the prostate-specific antigen gene as a reporter. Clin. Cancer Res. 10, (20), 6977-6984 (2004).
  9. Wu, Q., Mahendran, R., Esuvaranathan, K. Nonviral cytokine gene therapy on an orthotopic bladder cancer model. Clin. Cancer Res. 9, (12), 4522-4528 (2003).
  10. Bonfil, R. D., Russo, D. M., Binda, M. M., Delgado, F. M., Vincenti, M. Higher antitumor activity of vinflunine than vinorelbine against an orthotopic murine model of transitional cell carcinoma of the bladder. Urol. Oncol. 7, (4), 159-166 (2002).
  11. Bohle, A., Jurczok, A., et al. Inhibition of bladder carcinoma cell adhesion by oligopeptide combinations in vitro and in. 167, (1), 357-363 (2002).
  12. Gunther, J. H., Jurczok, A., et al. Optimizing syngeneic orthotopic murine bladder cancer (MB49). Cancer Res. 59, (12), 2834-2837 (1999).
  13. Gunther, J. H., Frambach, M., et al. Effects of acetylic salicylic acid and pentoxifylline on the efficacy of intravesical BCG therapy in orthotopic murine bladder cancer (MB49). J. Urol. 161, (5), 1702-1706 (1999).
  14. Dobek, G. L., Godbey, W. T. An orthotopic model of murine bladder cancer. J Vis Exp. (48), (2011).
  15. Tham, S. M., Ng, K. H., Pook, S. H., Esuvaranathan, K., Mahendran, R. Tumor and microenvironment modification during progression of murine orthotopic bladder cancer. Clin. Dev. Immunol. 2011, 865684 (2011).
  16. Seow, S. W., Cai, S., et al. Lactobacillus rhamnosus GG induces tumor regression in mice bearing orthotopic bladder tumors. Cancer Sci. 101, (3), 751-758 (2009).
  17. Mangsbo, S. M., Ninalga, C., Essand, M., Loskog, A., Totterman, T. H. CpG therapy is superior to BCG in an orthotopic bladder cancer model and generates CD4+ T-cell immunity. J. Immunother. 31, (1), 34-42 (2008).
  18. Loskog, A. S., Fransson, M. E., Totterman, T. T. AdCD40L gene therapy counteracts T regulatory cells and cures aggressive tumors in an orthotopic bladder cancer model. Clin. Cancer Res. 11, (24 Pt 1), 8816-8821 (2005).
  19. Loskog, A., Ninalga, C., et al. Optimization of the MB49 mouse bladder cancer model for adenoviral gene therapy. Lab Anim. 39, (4), 384-393 (2005).
  20. Bockholt, N. A., Knudson, M. J., et al. Anti-Interleukin-10R1 Monoclonal Antibody Enhances Bacillus Calmette-Guerin Induced T-Helper Type 1 Immune Responses and Antitumor Immunity in a Mouse Orthotopic Model of Bladder Cancer. J. Urol. 187, (6), 2228-2235 (2012).
  21. Watanabe, F. T., Chade, D. C., et al. Curcumin, but not Prima-1, decreased tumor cell proliferation in the syngeneic murine orthotopic bladder tumor model. Clinics. 66, (12), 2121-2124 (2011).
  22. Chade, D. C., Andrade, P. M., et al. Histopathological characterization of a syngeneic orthotopic murine bladder cancer model. Int. Braz. J. Urol. 34, (2), 220-226 (2008).
  23. Luo, Y., Chen, X., O'Donnell, M. A. Use of prostate specific antigen to measure bladder tumor growth in a mouse orthotopic model. J. Urol. 172, 2414-2420 (2004).
  24. Zhang, Z., Xu, X., et al. The therapeutic potential of SA-sCD40L in the orthotopic model of superficial bladder cancer. Acta Oncol. 50, (7), 1111-1118 (2011).
  25. Kohno, S., Luo, C., et al. Herpes simplex virus type 1 mutant HF10 oncolytic viral therapy for bladder cancer. Urology. 66, (5), 1116-1121 (2005).
  26. Kikuchi, E., Menendez, S., et al. Highly efficient gene delivery for bladder cancers by intravesically administered replication-competent retroviral vectors. Clin. Cancer Res. 13, (15 Pt 1), 4511-4518 (2007).
  27. Siemens, D. R., Austin, J. C., See, W. A., Tartaglia, J., Ratliff, T. L. Evaluation of gene transfer efficiency by viral vectors to murine bladder epithelium. J. Urol. 165, (2), 667-671 (2001).
  28. Siemens, D. R., Crist, S., Austin, J. C., Tartaglia, J., Ratliff, T. L. Comparison of viral vectors: gene transfer efficiency and tissue specificity in a bladder cancer model. J. Urol. 170, (3), 979-984 (2003).
  29. Black, P. C., Shetty, A., et al. Validating bladder cancer xenograft bioluminescence with magnetic resonance imaging: the significance of hypoxia and necrosis. BJU Int. 106, (11), 1799-1804 (2010).
जीन डिलीवरी के अध्ययन के लिए एक orthotopic मूत्राशय कैंसर के मॉडल
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Cite this Article

Kasman, L., Voelkel-Johnson, C. An Orthotopic Bladder Cancer Model for Gene Delivery Studies. J. Vis. Exp. (82), e50181, doi:10.3791/50181 (2013).More

Kasman, L., Voelkel-Johnson, C. An Orthotopic Bladder Cancer Model for Gene Delivery Studies. J. Vis. Exp. (82), e50181, doi:10.3791/50181 (2013).

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