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Simultânea Multicolor Imagem de Estruturas Biológicas com fluorescência Fotoativação Localização Microscopia

doi: 10.3791/50680 Published: December 9, 2013
* These authors contributed equally


Name Company Catalog Number Comments
LabTek II chambers Nunc
Fluorescent beads Invitrogen F-8801 Beads for calibration
Tetraspeck beads Invitrogen T-7279 Four color beads for calibration
Objective immersion oil Zeiss 518F Immersion oil for high NA objective (dependent on choice of objective)
HPLC water Fisher Scientific W5-4
Media ATCC 30-2003 Or Cellgro 10-090
Antibiotics GIBCO 15070-063
serum Thermo Scientific SH30087.03
Lipofectamine Invitrogen 52887
Optimem I GIBCO 11058-021
Trypsin MPBiomedicals 1689149
paraformaldehyde Fisher Scientific AA433689M CAUTION: Toxic



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Simultânea Multicolor Imagem de Estruturas Biológicas com fluorescência Fotoativação Localização Microscopia
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Cite this Article

Curthoys, N. M., Mlodzianoski, M. J., Kim, D., Hess, S. T. Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy. J. Vis. Exp. (82), e50680, doi:10.3791/50680 (2013).More

Curthoys, N. M., Mlodzianoski, M. J., Kim, D., Hess, S. T. Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy. J. Vis. Exp. (82), e50680, doi:10.3791/50680 (2013).

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