Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Click here for the English version

Biology

Gebruik doi: 10.3791/50840 Published: December 18, 2013

Materials

Name Company Catalog Number Comments
Antibody staning and fiaxtion paraformaldehyde Merc 8187151000
Tween-20  Bio-Rad 1706531
Collagenase Sigma C5138-100MG
DABCO Merc 8034560100
Triton X-100 Alfa Aesar A16046
β -ME Sigma 8057400250
BSA Di Cam 000-40-100
 Partial digest  Chymotrypsin Sigma C4129-250MG
Thermo-resistance SYTOX Orange Invitrogen S11368
Cholesterol Amaresco 0433-250G
EQUIPMENT
M165 FC fluorescent stereoscope Leica TXR filter
EXi Blue Fluorescence Microscopy Camera QImaging EXI-BLU-R-F-M-14-C
ConfoCor 3/510 META confocal microscope Zeiss
Pellet pestles Sigma Z359947-100EA
Pellet Pestle Cordless Motor Kontes K749540-0000
MicroCL 17 Microcentrifuge Series Thermo 75002455 Refrigerated, , 230 V 50/60 Hz, includes 24x 1.5/2.0 ml rotor with ClickSeal Biocontainment Lid

DOWNLOAD MATERIALS LIST

References

  1. Balch, W. E., Morimoto, R. I., Dillin, A., Kelly, J. W. Adapting proteostasis for disease intervention. Science. 319, 916-919 (2008).
  2. Arias, E., Cuervo, A. M. Chaperone-mediated autophagy in protein quality control. Curr. Opin. Cell. Biol. 23, 184-189 (2011).
  3. Tyedmers, J., Mogk, A., Bukau, B. Cellular strategies for controlling protein aggregation. Nat. Rev. Mol. Cell. Biol. 11, 777-788 (2010).
  4. Walter, P., Ron, D. The unfolded protein response: from stress pathway to homeostatic regulation. Science. 334, 1081-1086 (2011).
  5. Finley, D. Recognition and processing of ubiquitin-protein conjugates by the proteasome. Annu. Rev. Biochem. 78, 477-513 (2009).
  6. Gidalevitz, T., Prahlad, V., Morimoto, R. I. The stress of protein misfolding: from single cells to multicellular organisms. Cold Spring Harb Perspect Biol. 3, (2011).
  7. Haynes, C. M., Ron, D. The mitochondrial UPR - protecting organelle protein homeostasis. J. Cell. Sci. 123, 3849-3855 (2010).
  8. Akerfelt, M., Morimoto, R. I., Sistonen, L. Heat shock factors: integrators of cell stress, development and lifespan. Nat. Rev. Mol. Cell. Biol.. 11, 545-555 (2010).
  9. Prahlad, V., Morimoto, R. I. Integrating the stress response: lessons for neurodegenerative diseases from C. elegans. Trends Cell. Biol. 19, 52-61 (2009).
  10. Li, J., Soroka, J., Buchner, J. The Hsp90 chaperone machinery: conformational dynamics and regulation by co-chaperones. Biochim. Biophys. Acta. 1823, 624-635 (2012).
  11. Hartl, F. U., Bracher, A., Hayer-Hartl, M. Molecular chaperones in protein folding and proteostasis. Nature. 475, 324-332 (2011).
  12. Winkler, J., Tyedmers, J., Bukau, B., Mogk, A. Chaperone networks in protein disaggregation and prion propagation. J. Struct. Biol. 179, 152-160 (2012).
  13. Mayer, M. P. Gymnastics of molecular chaperones. Mol. Cell. 39, 321-331 (2010).
  14. Durieux, J., Wolff, S., Dillin, A. The cell-non-autonomous nature of electron transport chain-mediated longevity. Cell. 144, 79-91 (2011).
  15. Garcia, S. M., Casanueva, M. O., Silva, M. C., Amaral, M. D., Morimoto, R. I. Neuronal signaling modulates protein homeostasis in Caenorhabditis elegans post-synaptic muscle cells. Genes Dev. 21, 3006-3016 (2007).
  16. Prahlad, V., Cornelius, T., Morimoto, R. I. Regulation of the cellular heat shock response in Caenorhabditis elegans by thermosensory neurons. Science. 320, 811-814 (2008).
  17. Bar-Lavan, Y., Kosolapov, L., Frumkin, A., Ben-Zvi, A. Regulation of cellular protein quality control networks in a multicellular organism. FEBS J. 279, 526-531 (2012).
  18. Gupta, R., et al. Firefly luciferase mutants as sensors of proteome stress. Nat. Methods. 8, 879-884 (2011).
  19. Rampelt, H., et al. Metazoan Hsp70 machines use Hsp110 to power protein disaggregation. EMBO J. 31, 4221-4235 (2012).
  20. Winkler, J., Tyedmers, J., Bukau, B., Mogk, A. Hsp70 targets Hsp100 chaperones to substrates for protein disaggregation and prion fragmentation. J. Cell. Biol. 198, 387-404 (2012).
  21. Kim, Y. I., Burton, R. E., Burton, B. M., Sauer, R. T., Baker, T. A. Dynamics of substrate denaturation and translocation by the ClpXP degradation machine. Mol. Cell. 5, 639-648 (2000).
  22. Hamer, G., Matilainen, O., Holmberg, C. I. A photoconvertible reporter of the ubiquitin-proteasome system in vivo. Nat. Methods. 7, 473-478 (2010).
  23. Bence, N. F., Sampat, R. M., Kopito, R. R. Impairment of the ubiquitin-proteasome system by protein aggregation. Science. 292, 1552-1555 (2001).
  24. Dantuma, N. P., Lindsten, K., Glas, R., Jellne, M., Masucci, M. G. Short-lived green fluorescent proteins for quantifying ubiquitin/proteasome-dependent proteolysis in living cells. Nat. Biotechnol. 18, 538-543 (2000).
  25. Brown, C. R., Hong-Brown, L. Q., Welch, W. J. Correcting temperature-sensitive protein folding defects. J. Clin. Invest. 99, 1432-1444 (1997).
  26. Van Dyk, T. K., Gatenby, A. A., LaRossa, R. A. Demonstration by genetic suppression of interaction of GroE products with many proteins. Nature. 342, 451-453 (1989).
  27. Gidalevitz, T., Ben-Zvi, A., Ho, K. H., Brignull, H. R., Morimoto, R. I. Progressive disruption of cellular protein folding in models of polyglutamine diseases. Science. 311, 1471-1474 (2006).
  28. Ben-Zvi, A., Miller, E. A., Morimoto, R. I. Collapse of proteostasis represents an early molecular event in Caenorhabditis elegans aging. Proc. Natl. Acad. Sci. U.S.A. 106, 14914-14919 (2009).
  29. Burkewitz, K., Choe, K., Strange, K. Hypertonic stress induces rapid and widespread protein damage in. C. elegans. Am. J. Physiol. Cell Physiol. 301, 566-576 (2011).
  30. Alavez, S., Vantipalli, M. C., Zucker, D. J., Klang, I. M., Lithgow, G. J. Amyloid-binding compounds maintain protein homeostasis during ageing and extend lifespan. Nature. 472, 226-229 (2011).
  31. David, D. C., et al. Widespread protein aggregation as an inherent part of aging in C. elegans. PLoS Biol. 8, (2010).
  32. Eremenko, E., Ben-Zvi, A., Morozova-Roche, L. A., Raveh, D. Aggregation of human S100A8 and S100A9 amyloidogenic proteins perturbs proteostasis in a yeast model. PLoS One. In press, (2013).
  33. Gidalevitz, T., Krupinski, T., Garcia, S. M., Morimoto, R. I. Destabilizing protein polymorphisms in the genetic background direct phenotypic expression of mutant SOD1 toxicity. PLoS Genet. 5, (2009).
  34. Silva, M. C., et al. A genetic screening strategy identifies novel regulators of the proteostasis network. PLoS Genet.. 7, (2011).
  35. Prahlad, V., Morimoto, R. I. Neuronal circuitry regulates the response of Caenorhabditis elegans to misfolded proteins. Proc. Natl. Acad. Sci. U.S.A. 108, 14204-14209 (2011).
  36. Stiernagle, T. Maintenance of C. elegans. WormBook. 1-11 (2006).
  37. Gengyo-Ando, K., Kagawa, H. Single charge change on the helical surface of the paramyosin rod dramatically disrupts thick filament assembly in Caenorhabditis elegans. J. Mol. Biol. 219, 429-441 (1991).
  38. Clark, S. G., Shurland, D. L., Meyerowitz, E. M., Bargmann, C. I., vander Bliek, A. M. A dynamin GTPase mutation causes a rapid and reversible temperature-inducible locomotion defect in C. elegans. Proc. Natl. Acad. Sci. U.S.A.. 94, 10438-10443 (1997).
  39. Hsu, A. L., Murphy, C. T., Kenyon, C. Regulation of aging and age-related disease by DAF-16 and heat-shock factor. Science. 300, 1142-1145 (2003).
  40. Morley, J. F., Morimoto, R. I. Regulation of longevity in Caenorhabditis elegans by heat shock factor and molecular chaperones. Mol. Biol. Cell. 15, 657-664 (2004).
  41. Morimoto, R. I. Proteotoxic stress and inducible chaperone networks in neurodegenerative disease and aging. Genes Dev. 22, 1427-1438 (2008).
  42. Taylor, R. C., Dillin, A. Aging as an event of proteostasis collapse. Cold Spring Harb. Perspect. Biol.. 3, (2011).
  43. Tissenbaum, H. A. Genetics, Life Span, Health Span, and the Aging Process in Caenorhabditis elegans. J. Gerontol. A. Biological Sci. Med. Sci. 67, 503-510 (2012).
  44. Hosono, R., Mitsui, Y., Sato, Y., Aizawa, S., Miwa, J. Life span of the wild and mutant nematode Caenorhabditis elegans. Effects of sex, sterilization, and temperature. Exp. Gerontol. 17, 163-172 (1982).
  45. Madi, A., et al. Mass spectrometric proteome analysis for profiling temperature-dependent changes of protein expression in wild-type Caenorhabditis elegans. Proteomics. 3, 1526-1534 (2003).
  46. Herndon, L. A., et al. Stochastic and genetic factors influence tissue-specific decline in ageing C. elegans. Nature. 419, 808-814 (2002).
  47. Barral, J. M., Hutagalung, A. H., Brinker, A., Hartl, F. U., Epstein, H. F. Role of the myosin assembly protein UNC-45 as a molecular chaperone for myosin. Science. 295, 669-671 (2002).
  48. Melkani, G. C., Bodmer, R., Ocorr, K., Bernstein, S. I. The UNC-45 chaperone is critical for establishing myosin-based myofibrillar organization and cardiac contractility in the Drosophila heart model. PLoS One. 6, (2011).
  49. Gosai, S. J., et al. Automated high-content live animal drug screening using C. elegans expressing the aggregation prone serpin alpha1-antitrypsin Z. PLoS One. 5, (2010).
  50. Cohen, E., Bieschke, J., Perciavalle, R. M., Kelly, J. W., Dillin, A. Opposing activities protect against age-onset proteotoxicity. Science. (313), 1604-1610 (2006).
  51. Cohen, E., et al. Reduced IGF-1 signaling delays age-associated proteotoxicity in mice. Cell. 139, 1157-1169 (2009).
  52. Shemesh, N., Shai, N. Ben-Zvi A (2013) Germline stem cell arrest inhibits the collapse of somatic proteostasis early in Caenorhabditis elegans adulthood. Aging Cell. 12, (2013), 814-822 (2013).
Gebruik<em&gt; Caenorhabditis elegans</em&gt; Als een modelsysteem voor de Protein Homeostase studeren in een meercellige
Play Video
PDF DOI DOWNLOAD MATERIALS LIST

Cite this Article

Karady, I., Frumkin, A., Dror, S., Shemesh, N., Shai, N., Ben-Zvi, A. Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism. J. Vis. Exp. (82), e50840, doi:10.3791/50840 (2013).More

Karady, I., Frumkin, A., Dror, S., Shemesh, N., Shai, N., Ben-Zvi, A. Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism. J. Vis. Exp. (82), e50840, doi:10.3791/50840 (2013).

Less
Copy Citation Download Citation Reprints and Permissions
View Video

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter