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使用FURA - 2上午皮层神经元的钙成像
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Biology
Calcium Imaging of Cortical Neurons using Fura-2 AM
Please note that all translations are automatically generated.
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使用FURA - 2上午皮层神经元的钙成像
DOI:
10.3791/1067-v
•
12:15 min
•
January 19, 2009
•
Odmara L Barreto-Chang
,
Ricardo E Dolmetsch
1
Department of Neurobiology
,
Stanford University
,
2
Department of Neurobiology
,
Stanford University School of Medicine
Chapters
00:00
Title
00:53
Introduction
01:15
Loading Cells with Fura-2 Calcium Indicator Dye
05:02
Calcium Imaging of Cells
09:27
Defining and Analyzing Regions of Interest (ROI)
10:29
Representative Results/Outcome
11:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
钙信号,发挥了关键作用,在许多细胞过程,包括基因表达,存活和分化。在这里,我们将演示如何执行使用FURA - 2的钙成像。钙成像是一种宝贵的的工具来研究细胞内钙离子的实时调控及其信号传导的调控。
Tags
Calcium Imaging
Cortical Neurons
Fura-2 AM
Calcium Signals
Calcium Indicator Dyes
BAPTA-based Organic Molecules
Ratio-metric Dyes
Single-wavelength Dyes
Fluo-4
Intracellular Calcium Concentration
Fluorescence Emission
Excitation Spectra
Calcium Binding
Calcium Elevations
Excitable Cells
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