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使用FURA - 2上午皮层神经元的钙成像
JoVE Journal
Biology
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JoVE Journal Biology
Calcium Imaging of Cortical Neurons using Fura-2 AM
DOI:

12:15 min

January 19, 2009

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Chapters

  • 00:00Title
  • 00:53Introduction
  • 01:15Loading Cells with Fura-2 Calcium Indicator Dye
  • 05:02Calcium Imaging of Cells
  • 09:27Defining and Analyzing Regions of Interest (ROI)
  • 10:29Representative Results/Outcome
  • 11:30Conclusion

Summary

Automatic Translation

钙信号,发挥了关键作用,在许多细胞过程,包括基因表达,存活和分化。在这里,我们将演示如何执行使用FURA - 2的钙成像。钙成像是一种宝贵的的工具来研究细胞内钙离子的实时调控及其信号传导的调控。

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