/

/

视网膜水平切片制备

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Horizontal Slice Preparation of the Retina

Please note that all translations are automatically generated. Click here for the English version.

视网膜水平切片制备

DOI:

•

10:45 min

•

November 20, 2006

•

Ryosuke Enoki, Tatjana C. Jakobs, Amane Koizumi

¹Dpt of Physiology and Biophysics,Dalhousie University, ²Massachusetts General Hospital,Harvard Medical School

Chapters

00:05Tools
00:22Preparation of agar block and agarose gel
00:51Isolation of retina
02:06Preparation of retina slices
10:17Examples: cell layer of retina

Summary

Automatic Translation

传统的垂直切片和视网膜的整个安装准备视网膜电路的功能已被用来研究。在这里，我们描述了新的切割方法，保存完整的视网膜神经细胞的树突状形态。

Tags

Horizontal Slice Retina Retinal Circuits Amacrine Cells Dendrites Vertical Slice Whole-mount Preparation Morphology Patch-clamp Recordings Glial Cells Mueller Cells Slicing Method Vibratome Slicer Low-temperature Melting Agarose Gel Function Of Retinal Neurons Calcium Imaging Technique Immunocytochemistry Single-cell RT-PCR

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

光/暗转换测试小鼠

器官型培养的成年兔视网膜

薄切片进行免疫组化切片的筹备工作

器官型E18大鼠脑切片文化

基因枪子弹的制备和神经元的基因枪转染切片文化

全细胞记录的光在视网膜切片诱发兴奋性突触电流

眼内容剜除小鼠玻璃体和视网膜蛋白质组分析

在体内电穿孔Morpholinos到成年斑马鱼视网膜

在OVO在鸡胚视网膜电穿孔

切片培养法继牙胚在植文化的发展

Read Article