34,496 Views
•
07:03 min
•
July 02, 2009
DOI:
Summary
Automatically generated
Dissocier les cellules de différents types de tissus spécifiques requiert des paramètres spécifiques à l'agitation des tissus pour obtenir un volume élevé de viabilité, de cellules cultivables. Le Miltenyi gentleMACS dissociateur optimise cette tâche avec un simple, protocole pratique. Dans cette publication, l'utilisation de cet appareil sur le tissu pulmonaire est expliqué.
Related Videos
09:04
Isolation of Mouse Lung Dendritic Cells
Related Videos
28992 Views
05:18
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Related Videos
37665 Views
08:46
A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry
Related Videos
11772 Views
11:17
Generation of Single-Cell Suspensions from Mouse Neural Tissue
Related Videos
22451 Views
16:55
Isolation & Characterization of Hoechstlow CD45negative Mouse Lung Mesenchymal Stem Cells
Related Videos
20373 Views
09:15
Identification of Rare Antigen-Specific T Cells from Mouse Lungs with Peptide:Major Histocompatibility Complex Tetramers
Related Videos
857 Views
06:02
Isolation of Live Myeloid and Epithelial Cell Populations from the Mouse Lung
Related Videos
452 Views
09:47
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
Related Videos
8292 Views
04:03
Mouse Wound Models and Preparation of Single-Cell Suspensions
Related Videos
418 Views
07:55
Isolation of Lung Retinoid-Containing Cells by Cell Sorting
Related Videos
161 Views
Read Article
Cite this Article
Jungblut, M., Oeltze, K., Zehnter, I., Hasselmann, D., Bosio, A. Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator. J. Vis. Exp. (29), e1266, doi:10.3791/1266 (2009).
Copy