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July 02, 2009
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Summary
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Distanziert Zellen aus bestimmten Gewebearten erfordert spezifische Parameter für die Gewebe-Agitation um ein hohes Volumen an lebensfähigen, kultivierbaren Zellen zu erhalten. Die Miltenyi gentleMACS dissociator optimiert diese Aufgabe mit einem einfachen, praktischen Protokoll. In dieser Veröffentlichung wird die Verwendung dieser Vorrichtung auf das Lungengewebe erklärt.
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Cite this Article
Jungblut, M., Oeltze, K., Zehnter, I., Hasselmann, D., Bosio, A. Standardized Preparation of Single-Cell Suspensions from Mouse Lung Tissue using the gentleMACS Dissociator. J. Vis. Exp. (29), e1266, doi:10.3791/1266 (2009).
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