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Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain
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Batch Immunostaining for Large-Scale Protein Detection in the Whole Monkey Brain

Article DOI: 10.3791/1286-v • 08:08 min • July 27th, 2009
July 27th, 2009 •
1,2, 2, 3, 2
1Cognitive Neuroscience Unit, Montreal Neurological Institute, 2Ècole d’Optomètrie, Universitè de Montrèal, 3Department of Psychology, McGill University

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Large-scale immunodetection of target proteins across the entire primate brain is possible by employing novel tissue embedding and sectioning methods combined with the use of creative apparatus for batch staining of multiple free-floating sections at a given time.

Tags

Batch Immunostaining Large-scale Protein Detection Whole Monkey Brain Immunohistochemistry (IHC) Target Proteins Expression Pattern Brain Rodents Primates Systematic Approach Immunodetection Adult Monkey Brain Tissue Embedding Sectioning Methodology Neuroscience Associates (NSA) Batch-staining Free-floating Sections Uniform Staining Analytical Procedures Protein Levels Neurons Expressing Protein
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