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Chapters
- 01:07Weaknesses of in vivo angiogenesis studies
- 01:56Advantages of the fibrin gel/bead assay
- 04:10Why use HUVEC (Human Umbilical Vein Endotheleial Cells)
- 05:47Quantifying angiogenesis in vitro
- 06:30Significance of fibroblasts
- 07:21Angiogenic factors secreted by fibroblasts
- 08:57Angiogenic factors in vivo vs in vitro
Christopher C.W. Hughes describes the utility of his culture system for studying angiogenesis in vitro. He explains the importance of fibroblasts that secrete a critical, yet unidentified, soluble factor that allow endothelial cells to form vessels in culture that branch, form proper lumens, and undergo anastamosis.
