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JoVE Journal
Biology

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Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
 
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Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

Article DOI: 10.3791/1842-v 07:57 min December 3rd, 2009
December 3rd, 2009

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Summary

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Click here for the English version.

The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.

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GELFREE 8100 Fractionation System Protein Fractionation Molecular Weight-based Fractionation Liquid Phase Recovery Single-use Cartridges GELFREE Fractionation Instrument Constant Voltage Electrophoretic Mobility Resolving Gel Stacking Gel Collection Chamber Fractions Collection Pipette Complex Protein Mixtures Total Protein Per Channel
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