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05:28 min
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April 29, 2007
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Summary
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This video demonstrates the preparation of an ultra-thin matrix/analyte layer for analyzing peptides and proteins by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS).
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MALDISample PreparationUltra Thin Layer MethodMatrix-Assisted Laser Desorption Ionization Mass SpectrometryMatrix CrystalsAlpha-cyano-4-hydroxycinnamic AcidSubstrate LayerCrystallizationAdvantagesGreater Tolerance To ImpuritiesBetter ResolutionHigher Spatial UniformityAccurate Mass DeterminationMembrane ProteinsIon ChannelsMetabolite TransportersReceptorsTransmembrane DomainsSoluble ProteinsMolecular MassesHigh-quality Spectra
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Cite this Article
Fenyo, D., Wang, Q., DeGrasse, J. A., Padovan, J. C., Cadene, M., Chait, B. T. MALDI Sample Preparation: the Ultra Thin Layer Method. J. Vis. Exp. (3), e192, doi:10.3791/192 (2007).
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