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Isolering och In vitro Aktivering av Caenorhabditis elegans Spermier
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and In vitro Activation of Caenorhabditis elegans Sperm
DOI:

05:46 min

January 31, 2011

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Chapters

  • 00:05Title
  • 00:21Subscription
  • 00:40Introduction
  • 01:29Enrichment of Male Population, Identification, and Isolation of Males
  • 02:29Dissection of Males and in vitro Activation
  • 04:25Visualization of Spermtids and Spermatozoa
  • 04:54Images of C. elegans Spermatid Isolation and Activation
  • 05:21Conclusion

Summary

Automatic Translation

Ett protokoll för isolering och aktivera spermatider från manliga<em> C. elegans</em> Beskrivs här. Kapning den bakre änden av manliga releaser spermatider. Den spermatider kan aktiveras genom tillsats av proteashämmare.

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