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JoVE Journal Neuroscience
Produktion av Chick Embryo Extract för odling av murina neurala celler Crest Stem
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Produktion av Chick Embryo Extract för odling av murina neurala celler Crest Stem

Article DOI: 10.3791/2380-v • 10:12 min • November 27th, 2010
November 27th, 2010 •
*1, *1, 2, 2, 1, 1, 1
1Department of Pediatric Oncology, University Children's Hospital Essen, 2Department of Developmental Pathology, Bonn Medical School, Institute of Pathology
* These authors contributed equally

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Att odla neurala celler crest stamceller (NCSC) in vitro, är ett speciellt medium (NCSCM) krävs. Viktig del av NCSCM är kycklingembryo extrakt (CEE). Vi beskriver här exakta tekniker för att producera en maximal mängd av ren och hög kvalitet CEE, däribland detaljer isolering, maceration, centrifugering och filtrering.

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Neurovetenskap cellbiologi möss neural crest stamceller kycklingembryo extrakt
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