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Immunology and Infection

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JoVE Journal Immunology and Infection
Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation
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Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

Article DOI: 10.3791/2381-v • 07:20 min • January 14th, 2011
January 14th, 2011 •
1, 2, 3, 3, 1
1Department of Microbiology & Immunology, Virginia Commonwealth University- Massey Cancer Center, 2Department of Internal Medicine, Virginia Commonwealth University- Massey Cancer Center, 3Department of Surgery, Virginia Commonwealth University- Massey Cancer Center

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An efficient protocol for the ex vivo expansion of tumor-reactive T cells from tumor-draining lymph nodes or other secondary lymphoid tissues of tumor-bearing hosts is described. This protocol selectively expands tumor-specific T cells for use in adoptive immunotherapy of breast cancer.

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Ex Vivo Expansion Tumor-reactive T Cells Bryostatin 1/Ionomycin Common Gamma Chain Cytokines Formulation Breast Cancer Immune Responses Adoptive Immunotherapy Tumor-specific T Cells Tumor Antigens IL-2 IL-7 IL-15 IL-21 FVBN202 Transgenic Mouse Model HER-2/neu Positive Breast Carcinoma Adoptive T Cell Therapy
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