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JoVE Journal
Biology

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Immunocytochemistry
 
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Immunocytochemistry: Human Neural Stem Cells

Article DOI: 10.3791/267-v 19:42 min August 24th, 2007
August 24th, 2007

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Summary

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Click here for the English version.

Immunocytochemistry is a powerful method to determine the presence, subcellular localization, and relative abundance of an antigen of interest in cultured cells. This protocol presents an easy-to-follow series of steps that will enable one to conserve antibodies and get the most out of one's staining.

Tags

Immunocytochemistry Human Neural Stem Cells Antigen Protein Cultured Cells Subcellular Localization Relative Abundance Antibodies Staining Protocol Qualitative Data Quantitative Data Coverslips Antibody Solution Parafilm Sample Immunostaining HNSPCs
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