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一个渐变产生的微流体装置,细胞生物学
JoVE Journal
Biology
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JoVE Journal
Biology
A Gradient-generating Microfluidic Device for Cell Biology
Please note that all translations are automatically generated.
Click here for the English version.
一个渐变产生的微流体装置,细胞生物学
DOI:
10.3791/271-v
•
11:05 min
•
August 30, 2007
•
Bong Geun Chung
,
Amir Manbachi
,
Wajeeh Saadi
,
Francis Lin
,
Noo Li Jeon
,
Ali Khademhosseini
1
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology; Center for Biomedical Engineering, Department of Medicine
,
Brigham and Women’s Hospital
Chapters
00:05
Introduction
00:21
Microfabrication of microfluidic device
00:56
Preparation of PDMS mixture
01:49
Patterning PDMS molds
02:20
Removing bubbles by vacuum
03:19
Assembly of microfluidic device
03:20
Cutting layers of microfluidic device
03:33
Creating channel inlet and outlet
03:59
Bonding the microfluidic device layers
06:55
Experiment: effect of EGF gradient on cell migration
07:13
Application of reagents
09:49
Experiment: cell migration
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们描述了一个协议梯度产生的微流体装置,可以产生良好定义的微环境中的空间和时间的梯度微细。在这种方法中,梯度产生的微流体装置可用于研究细胞定向迁移,胚胎发育,伤口愈合,癌症转移。
Tags
Gradient-generating Microfluidic Device
Cell Biology
Cellular Behavior
Microfluidic Platform
Fluid Flows
High-throughput Experiments
Soluble Concentration Gradients
PDMS-based Microfluidic Devices
Growth Factors
Fluorescein Isothyiocyanate (FITC)-dextran
Epidermal Growth Factor (EGF)
Fibroblasts
Cell Migration
Cell Motility
Cell Tracking Analysis
Article
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