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一个渐变产生的微流体装置,细胞生物学
JoVE Journal
Biology
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JoVE Journal Biology
A Gradient-generating Microfluidic Device for Cell Biology
DOI:

11:05 min

August 30, 2007

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Chapters

  • 00:05Introduction
  • 00:21Microfabrication of microfluidic device
  • 00:56Preparation of PDMS mixture
  • 01:49Patterning PDMS molds
  • 02:20Removing bubbles by vacuum
  • 03:19Assembly of microfluidic device
  • 03:20Cutting layers of microfluidic device
  • 03:33Creating channel inlet and outlet
  • 03:59Bonding the microfluidic device layers
  • 06:55Experiment: effect of EGF gradient on cell migration
  • 07:13Application of reagents
  • 09:49Experiment: cell migration

Summary

Automatic Translation

我们描述了一个协议梯度产生的微流体装置,可以产生良好定义的微环境中的空间和时间的梯度微细。在这种方法中,梯度产生的微流体装置可用于研究细胞定向迁移,胚胎发育,伤口愈合,癌症转移。

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