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Chapters
- 00:05Title
- 01:27Preparation of Protein Mixtures for Injection
- 03:11Cytoplasmic Microinjection of Cytochrome c
- 06:34Cytochrome c Injected Fibroblasts and Neurons
- 07:24Conclusion
In questo protocollo, si descrive la microiniezione diretta citoplasmatico del citocromo<em> C</emProteine> in fibroblasti e primario neuroni simpatici. Questa tecnica permette l'introduzione del citocromo<em> C</emProteine> nel citoplasma delle cellule e imita il rilascio del citocromo<em> C</em> Dai mitocondri, che si verifica durante l'apoptosi.
Tags
ApoptosisProgrammed Cell DeathCytoplasmic MicroinjectionCytochrome CMitochondrial Intermembrane SpaceCaspasesCytotoxic StressesSignaling CascadesHolocytochrome CHeme-attached ProteinPost-mitochondrial RegulationCDNA ConstructsCytosolic CaspasesPurified Heme-attached Cytochrome C ProteinMitochondrial Cytochrome C Release
