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Chapters
- 00:05Title
- 01:27Preparation of Protein Mixtures for Injection
- 03:11Cytoplasmic Microinjection of Cytochrome c
- 06:34Cytochrome c Injected Fibroblasts and Neurons
- 07:24Conclusion
Neste protocolo, descrevemos a microinjeção direta citoplasmática de citocromo<em> C</em> Proteína em fibroblastos e primário neurônios simpáticos. Esta técnica permite a introdução do citocromo<em> C</em> Proteínas no citoplasma das células e imita a liberação de citocromo<em> C</em> A partir de mitocôndrias, que ocorre durante a apoptose.
Tags
ApoptosisProgrammed Cell DeathCytoplasmic MicroinjectionCytochrome CMitochondrial Intermembrane SpaceCaspasesCytotoxic StressesSignaling CascadesHolocytochrome CHeme-attached ProteinPost-mitochondrial RegulationCDNA ConstructsCytosolic CaspasesPurified Heme-attached Cytochrome C ProteinMitochondrial Cytochrome C Release
