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Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures
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Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures

Article DOI: 10.3791/1347 • 08:22 min • May 20th, 2009
May 20th, 2009 •
1, 1, 2
1Department of Biological Sciences, University of Illinois, 2Department of Biological Sciences, Vanderbilt University

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Summary
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This technique exposes the Drosophila embryonic neuromusculature for immunohistochemistry or electrophysiological recording. It is useful for studying early events in neuromuscular development or performing electrophysiology in mutants that cannot hatch.

Tags

Drosophila Embryos Electrophysiological Recording Procedures Genetic Model Embryonic Development Functional Neuroscience Developmental Programs Electrical Signaling Properties Chemical Synapses Neural Circuit Formation Investigation Late Developmental Period Epidermal Cuticle Surgical Glue Controlled Dissection Dorsal Longitudinal Incision Ventral Nerve Cord Body Wall Musculature Genetic Mutants Synapse Formation Molecular Mechanisms
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