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测量心肌细胞的快速钙助焊剂
JoVE Journal
Biology
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JoVE Journal Biology
Measuring Fast Calcium Fluxes in Cardiomyocytes
DOI:

12:10 min

November 29, 2011

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Chapters

  • 00:05Title
  • 01:15Loading Cells with Calcium Indicators
  • 02:23Microinjecting Cells
  • 03:42Co-immunofluorescence Studies
  • 04:49Fast Calcium Imaging and Data Analysis
  • 06:28Dissecting the Timing of Ca++ Fluxes Facilitates Evaluation of Their Impact on Cellular Events
  • 11:22Conclusion

Summary

Automatic Translation

我们提出了一个方法来隔离在生活细胞利用激光扫描共聚焦显微镜慢通量快速(微秒)钙事件。方法措施,由几百个像素单元格中的记录行扫描荧光强度的钙指标的波动。直方图分析,让我们来隔离不同的钙通量的时间尺度。

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