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测量心肌细胞的快速钙助焊剂
JoVE Journal
Biology
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JoVE Journal
Biology
Measuring Fast Calcium Fluxes in Cardiomyocytes
Please note that all translations are automatically generated.
Click here for the English version.
测量心肌细胞的快速钙助焊剂
DOI:
10.3791/3505-v
•
12:10 min
•
November 29, 2011
•
Urszula Golebiewska
,
Suzanne Scarlata
1
Department of Biological Sciences and Geology
,
Queensborough Community College
,
2
Department of Physiology & Biophysics
,
Stony Brook University
Chapters
00:05
Title
01:15
Loading Cells with Calcium Indicators
02:23
Microinjecting Cells
03:42
Co-immunofluorescence Studies
04:49
Fast Calcium Imaging and Data Analysis
06:28
Dissecting the Timing of Ca++ Fluxes Facilitates Evaluation of Their Impact on Cellular Events
11:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个方法来隔离在生活细胞利用激光扫描共聚焦显微镜慢通量快速(微秒)钙事件。方法措施,由几百个像素单元格中的记录行扫描荧光强度的钙指标的波动。直方图分析,让我们来隔离不同的钙通量的时间尺度。
Tags
Calcium Fluxes
Cardiomyocytes
Measuring
Fast
Ca2+ Activity
Phospholipase Cβ- Gαq Pathway
Acetylcholine
Caveolae
Entrapment
Gαq
Prolonged Ca2+ Signals
Dynamic Calcium Responses
Fluorescent Ca2+ Indicator
Ca2+ Green
Fluorescence Emission Intensity
Line-scan Mode
Laser Scanning Confocal Microscope
Time Course
Microsecond Time Scale
Excel Analysis
Sigmaplot Analysis
Flux Rate
Article
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