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Chapters
- 00:05Title
- 01:15Loading Cells with Calcium Indicators
- 02:23Microinjecting Cells
- 03:42Co-immunofluorescence Studies
- 04:49Fast Calcium Imaging and Data Analysis
- 06:28Dissecting the Timing of Ca++ Fluxes Facilitates Evaluation of Their Impact on Cellular Events
- 11:22Conclusion
Vi præsenterer en metode til at isolere hurtig (mikrosekund) calcium begivenheder fra langsommere flusmidler i levende celler ved hjælp af laser scanning konfokal mikroskopi. Metoden foranstaltninger fluorescensintensitet udsving af calcium indikatorer ved at optage linje scanninger af flere hundrede pixel i en celle. Histogram analyse giver os mulighed for at isolere tidsskalaer af forskellige calcium flux.
Tags
Calcium FluxesCardiomyocytesMeasuringFastCa2+ ActivityPhospholipase Cβ- Gαq PathwayAcetylcholineCaveolaeEntrapmentGαqProlonged Ca2+ SignalsDynamic Calcium ResponsesFluorescent Ca2+ IndicatorCa2+ GreenFluorescence Emission IntensityLine-scan ModeLaser Scanning Confocal MicroscopeTime CourseMicrosecond Time ScaleExcel AnalysisSigmaplot AnalysisFlux Rate
