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JoVE Journal
Biology
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鉴定蛋白质相互作用的合作伙伴使用串联亲和纯化
 
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鉴定蛋白质相互作用的合作伙伴使用串联亲和纯化

Article DOI: 10.3791/3643
February 25th, 2012

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Summary February 25th, 2012

Please note that all translations are automatically generated.

Click here for the English version.

串联亲和纯化的蛋白结合合作伙伴的标识是稳健的做法。由于概念证明,这种方法的应用以及特色的翻译起始因子eIF4E共沉淀的宿主细胞因子参与翻译起始。这种方法是很容易适应任何细胞或病毒蛋白。

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