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一个协议,用于噬菌体展示和亲和选择以重组蛋白诱饵
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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Please note that all translations are automatically generated.
Click here for the English version.
一个协议,用于噬菌体展示和亲和选择以重组蛋白诱饵
DOI:
10.3791/50685-v
•
12:36 min
•
February 16, 2014
•
Rekha Kushwaha
,
Kim R. Schäfermeyer
,
A. Bruce Downie
1
Department of Horticulture
,
University of Kentucky
Chapters
00:05
Title
01:14
Growing Bacterial Host (BLT5403) for Titering
02:15
Affinity Selection
08:13
Titering
09:41
Plaque Isolation
10:59
Results: Typical Titer and PCR Results for Affinity Selection over an Appropriate Bait
12:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
噬菌体展示是一种强大的技术,以捕获与感兴趣的固定化分子相互作用的蛋白质或蛋白质的部分。一旦噬菌体cDNA文库的类型来创建和屏幕的决定已经作出,这里所描述的协议允许高效亲和选择,导致识别干扰作用的。
Tags
Phage Display
Affinity Selection
Recombinant Protein Baits
Protein-protein Interactions
CDNA Library
Affinity Selection Protocol
Phage Titer
Negative Controls
Amplicons
False Positives
Viral Contamination
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