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一个协议,用于噬菌体展示和亲和选择以重组蛋白诱饵
JoVE Journal
Biology
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JoVE Journal Biology
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
DOI:

12:36 min

February 16, 2014

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Chapters

  • 00:05Title
  • 01:14Growing Bacterial Host (BLT5403) for Titering
  • 02:15Affinity Selection
  • 08:13Titering
  • 09:41Plaque Isolation
  • 10:59Results: Typical Titer and PCR Results for Affinity Selection over an Appropriate Bait
  • 12:12Conclusion

Summary

Automatic Translation

噬菌体展示是一种强大的技术,以捕获与感兴趣的固定化分子相互作用的蛋白质或蛋白质的部分。一旦噬菌体cDNA文库的类型来创建和屏幕的决定已经作出,这里所描述的协议允许高效亲和选择,导致识别干扰作用的。

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