Biology
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的蛋白质相互作用的合作伙伴在哺乳动物细胞中使用SILAC-免疫定量蛋白质组学鉴定
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Summary
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SILAC免疫实验指用于发现新蛋白的有力手段:蛋白质相互作用。通过使蛋白质丰度的控制和测试样品,真相互作用的精确相对定量可以很容易地从实验的污染物区分开来,并且低亲和力相互作用,通过使用较少的严格的缓冲条件下保存。
Tags
生物化学,第89期,质谱法,组织培养技术,同位素标记,SILAC,氨基酸在细胞培养稳定同位素标记,蛋白质组学,Interactomics,免疫沉淀,下拉,eIF4A,绿色荧光蛋白,nanotrap,轨道阱Related Videos
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