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Immunohistological Mærkning af mikrotubuli i Sensorisk Neuron dendritter, tracheae, og muskler i Drosophila Larver Krop Wall
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Chapters
- 00:05Title
- 01:11Larval Dissection
- 02:56Fixation of Larval Fillets
- 03:59Blocking, Staining, and Mounting of Larval Fillets
- 05:56Fluorescent Staining
- 07:22Conclusion
For at forstå, hvordan komplekse celle former, såsom neuronal dendritter, er opnået under udviklingen, er det vigtigt at være i stand til præcist at analysen mikrotubuli organisation. Her beskriver vi en robust immunohistological mærkning metode til at undersøge mikrotubuli organisering af dendritiske arborization neuron sensoriske dendritter, luftrør, muskler og andre<em> Drosophila</em> Larve krop væg væv.
Tags
Immunohistological LabelingMicrotubulesSensory Neuron DendritesTracheaeMusclesDrosophila Larva Body WallCell ShapesMicrotubule OrganizationTube MorphogenesisDendritic ArborizationNeuron-class-specific MechanismsDendritic DifferentiationDegenerationCytoskeletal OrganizationImmunological Labeling MethodIn Vivo Microtubule OrganizationFirst Instar LarvaSensory Neuron Dendrite OutgrowthBranching OrganizationMuscles LabelingTrachea Labeling
