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Simulating Pancreatic Neuroplasticity: In Vitro Dual-neuron Plasticity Assay
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Simulating Pancreatic Neuroplasticity: In Vitro Dual-neuron Plasticity Assay

Simulating Pancreatic Neuroplasticity: In Vitro Dual-neuron Plasticity Assay

DOI:
10.3791/51049-v

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10:29 min

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April 14, 2014

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, , , ,
1Department of Surgery, Klinikum rechts der Isar,Technische Universität München, 2Department of Biotechnology,University of Applied Sciences Kaiserslautern/Zweibrücken

Chapters

  • 00:05Title
  • 01:47Collecting Tissue Extracts and Cell Line Supernatants
  • 03:48Isolation of the Dorsal Root Ganglia (DRG) Neurons
  • 05:35Culturing Neurons and Glia with Pancreatic Tissue Extracts and Cell-line Supernatants
  • 08:01Results: Neuronal and Glial Plasticity Due to Pancreatic Microenvironmental Cues
  • 09:37Conclusion

Summary

Automatic Translation

Automatic Translation

Neuronal plasticity is an increasingly recognized, but insufficiently understood feature of the gastrointestinal (GI) tract. Here, in the example of human pancreatic disorders, we present an in vitro neuroplasticity assay for the study of neuronal plasticity in the GI tract at both morphological and functional level.

Tags

Keywords: Pancreatic NeuroplasticityEnteric Nervous SystemGastrointestinal InnervationPancreatic CancerChronic PancreatitisDorsal Root GangliaMyenteric PlexusDual-neuron AssayIn Vitro ModelNeuronal MorphologyElectrophysiologyMicroenvironmental Modulation

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