Medicine
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Cholesterol Efflux Assay
Chapters
Summary
The cholesterol assay is designed to quantitate the rate of cholesterol efflux from cultured cells and the capacity of plasma acceptors to accept cholesterol released from cells. The assay consists of labelling cells with cholesterol, equilibration of cholesterol among intracellular pools and release of cholesterol to an extracellular acceptor.
Tags
Cholesterol Efflux Assay Cholesterol Content Cellular Membranes Apoptosis Necrosis Intracellular Synthesis Plasma Lipoproteins Cholesterol Synthesis Cholesterol Uptake Deficiencies Of Cholesterol Excessive Cholesterol Hepatocytes Adrenocortical Cells Cholesteryl Esters ATP Binding Cassette Transporter Proteins A1 (ABCA1) ATP Binding Cassette Transporter Proteins G1 (ABCG1) Scavenger Receptor Type B1 High Density Lipoprotein (HDL) Apolipoprotein A-IRelated Videos
PLAYLIST
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Education • Core: BiologyIntroduction to Membrane Proteins
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Education • Core: Molecular BiologyThe Equilibrium Binding Constant and Binding Strength
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Education • Core: Molecular BiologyGTPases and their Regulation
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Education • Core: ChemistryStructure of Lipids
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Research • BioengineeringFluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
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Research • EnvironmentExtraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
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Research • BiochemistryIsolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
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Research • BiochemistryQuantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
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Research • MedicineCholesterol Efflux Assay
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Research • MedicineDifferential Effects of Lipid-lowering Drugs in Modulating Morphology of Cholesterol Particles
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Research • BiologyLDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring
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Education • Core: BiologyFacilitated Transport
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Education • Core: BiologyPrimary Active Transport
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Education • Core: BiologySecondary Active Transport
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Education • Lab BioDiffusion and Osmosis - Student Protocol
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Education • Lab BioDiffusion and Osmosis - Prep Student