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Journal
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Medicine
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胆固醇外流检测
JoVE Journal
Medicine
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JoVE Journal
Medicine
Cholesterol Efflux Assay
Please note that all translations are automatically generated.
Click here for the English version.
胆固醇外流检测
DOI:
10.3791/3810-v
•
07:54 min
•
March 06, 2012
•
Hann Low
,
Anh Hoang
,
Dmitri Sviridov
1
Baker IDI Heart and Diabetes Institute
Chapters
00:05
Title
01:32
Preparation of 3H-cholesterol
01:53
Label Cellular Cholesterol
02:37
Equilibrium Incubation
03:19
Cholesterol Efflux Incubation
04:10
Processing Samples
05:45
Data Analysis
06:11
Representative Results: Assaying Modulators of Cholesterol Efflux in Macrophages
07:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
胆固醇检测到定量,从培养细胞的胆固醇流出率和血浆受体的能力,接受从细胞释放的胆固醇。该法由标记细胞内池和胆固醇释放到细胞外受体之间的胆固醇,胆固醇平衡。
Tags
Cholesterol Efflux Assay
Cholesterol Content
Cellular Membranes
Apoptosis
Necrosis
Intracellular Synthesis
Plasma Lipoproteins
Cholesterol Synthesis
Cholesterol Uptake
Deficiencies Of Cholesterol
Excessive Cholesterol
Hepatocytes
Adrenocortical Cells
Cholesteryl Esters
ATP Binding Cassette Transporter Proteins A1 (ABCA1)
ATP Binding Cassette Transporter Proteins G1 (ABCG1)
Scavenger Receptor Type B1
High Density Lipoprotein (HDL)
Apolipoprotein A-I
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