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March 05, 2012
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AVEXIS er et højt gennemløb protein-interaktion assay udviklet til systematisk screening for hidtil ukendte ekstracellulære receptor-ligand-par involveret i cellulære genkendelse processer. Det er specielt designet til at detektere transiente proteininteraktioner, der er vanskelige at identificere ved hjælp af andre højt gennemløb fremgangsmåder.
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Avidity-based Extracellular Interaction ScreeningAVEXISLow-affinity InteractionsExtracellular Receptor-ligand InteractionsIntercellular CommunicationMulticellular OrganismsProtein-protein InteractionsBinding PartnerMembrane-embedded ProteinsPosttranslational ModificationsInteraction AffinitiesHigh Throughput MethodsAVEXIS AssayWeak Protein InteractionsFalse Positive RateHigh Throughput FormatRecombinant Protein Libraries
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Kerr, J. S., Wright, G. J. Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions. J. Vis. Exp. (61), e3881, doi:10.3791/3881 (2012).
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