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1The Brady Urological Institute,Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering, School of Life Sciences,Ulsan National Institute of Science and Technology (UNIST), 3Department of Urology, Renji Hospital,Shanghai Jiao Tong University School of Medicine, 4Center for Soft and Living Matter,Institute for Basic Science (IBS)
Chapters
- 00:04Introduction
- 01:15Extracellular Vesicle (EV) Isolation and On-Chip Immuno-Fluorescent Labeling
- 02:54Incubation of the Cells with Fluorescently Labeled EVs for the EV-Uptake Assay
- 03:45Confocal Microscopy and Image Processing
- 06:28Results: EV Uptake Assay Using 3D Fluorescence Imaging via Confocal Microscopy
- 07:31Conclusion
Ekstracellulære vesikler (elbiler) bidrager til cellulær biologi og intercellulær kommunikation. Der er behov for praktiske analyser til at visualisere og kvantificere elbiler optagelse af cellerne. Den nuværende protokol foreslår EV optagelse assay ved hjælp af tre-dimensionelle fluorescens billeddannelse via konfokal mikroskopi, efter EV isolation af en nano-filtrering-baseret mikrofluidisk enhed.
